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Literature summary for 4.1.99.2 extracted from

  • Tang, X.L.; Liu, X.; Suo, H.; Wang, Z.C.; Zheng, R.C.; Zheng, Y.G.
    Process development for efficient biosynthesis of L-DOPA with recombinant Escherichia coli harboring tyrosine phenol lyase from Fusobacterium nucleatum (2018), Bioprocess Biosyst. Eng., 41, 1347-1354 .
    View publication on PubMed
Search for more literature for 4.1.99.2

Application

Application Comment Organism
synthesis the tyrosine phenol lyase (TPL) catalyzed synthesis of L-DOPA is regarded as one of the most economic routes for L-DOPA synthesis Fusobacterium nucleatum subsp. nucleatum

Cloned(Commentary)

Cloned (Comment) Organism
gene tnaA, recombinant expression in Escherichia coli strain BL21(DE3) Fusobacterium nucleatum subsp. nucleatum

Protein Variants

Protein Variants Comment Organism
additional information the tyrosine phenol lyase (TPL) catalyzed synthesis of L-DOPA is regarded as one of the most economic routes for L-DOPA synthesis, usage in a fed-batch approach with sodium pyruvate, dicatechol and ammonium acetate. In addition, L-DOPA crystals are exogenously added to inhibit cell encapsulation by the precipitated product. The final L-DOPA concentration reaches higher than 120 g/l with dicatechol conversion more than 96% in a 15-l stirred tank, demonstrating the great potential of Fn-TPL for industrial production of L-DOPA. Optimization of reaction conditions and upscaling of the recombinant enzyme TPL from Fusobacterium nucleatum in enzyme-expressing Escherichia coli cells, detailed overview Fusobacterium nucleatum subsp. nucleatum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-tyrosine + H2O Fusobacterium nucleatum subsp. nucleatum
-
 phenol + pyruvate + NH3
-
 r
L-tyrosine + H2O Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131
-
 phenol + pyruvate + NH3
-
 r

Organism

Organism UniProt Comment Textmining
Fusobacterium nucleatum subsp. nucleatum Q8RHM6
-
-
Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131 Q8RHM6
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain BL21(DE3) Fusobacterium nucleatum subsp. nucleatum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tyrosine + H2O
-
 Fusobacterium nucleatum subsp. nucleatum phenol + pyruvate + NH3
-
 r
L-tyrosine + H2O
-
 Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131 phenol + pyruvate + NH3
-
 r
pyrocatechol + pyruvate + NH4+
-
 Fusobacterium nucleatum subsp. nucleatum L-DOPA + H2O
-
 r
pyrocatechol + pyruvate + NH4+
-
 Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131 L-DOPA + H2O
-
 r

Synonyms

Synonyms Comment Organism
Fn-TPL
-
 Fusobacterium nucleatum subsp. nucleatum
TnaA
-
 Fusobacterium nucleatum subsp. nucleatum
TPL
-
 Fusobacterium nucleatum subsp. nucleatum
tyrosine phenol lyase
-
 Fusobacterium nucleatum subsp. nucleatum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
15
-
 recombinant enzyme, L-DOPA synthesis in recombinant Escherichia coli cells Fusobacterium nucleatum subsp. nucleatum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 recombinant enzyme, L-DOPA synthesis in recombinant Escherichia coli cells Fusobacterium nucleatum subsp. nucleatum

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate dependent on Fusobacterium nucleatum subsp. nucleatum