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Literature summary for 4.1.99.22 extracted from

  • Mehta, A.P.; Hanes, J.W.; Abdelwahed, S.H.; Hilmey, D.G.; Haenzelmann, P.; Begley, T.P.
    Catalysis of a new ribose carbon-insertion reaction by the molybdenum cofactor biosynthetic enzyme MoaA (2013), Biochemistry, 52, 1134-1136.
    View publication on PubMedView publication on EuropePMC

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ MoaA harbors two [4Fe-4S]2+,1+ clusters, the N-terminal [4Fe-4S] cluster is likely to be responsible for the reductive cleavage of SAM to the 5'-deoxyadenosyl radical and L-methionine and the second cluster binds to N1 of GTP Bacteria

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP Bacteria
-
cyclic pyranopterin phosphate + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Bacteria
-
-
-

Reaction

Reaction Comment Organism Reaction ID
GTP + S-adenosyl-L-methionine + reduced electron acceptor = (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + oxidized electron acceptor the N-terminal [4Fe-4S] cluster is likely to be responsible for the reductive cleavage of SAM to the 5'-deoxyadenosyl radical and L-methionine and the second cluster binds to N1 of GTP. Upon generation of the 5'-deoxyadenosyl radical, GTP is transformed into (2-amino-7-hydroxy-4,6-dioxo-4,5,5a,6,7,8,9a,10-octahydro-3H-pyrano[3,2-g]pteridin-8-yl)methyl triphosphate via a complex rearrangement reaction where the C8 atom of the purine is inserted between the C2' and the C3' atoms of the ribose moiety. MoaC then catalyzes the intramolecular cyclization reaction of (2-amino-7-hydroxy-4,6-dioxo-4,5,5a,6,7,8,9a,10-octahydro-3H-pyrano[3,2-g]pteridin-8-yl)methyl triphosphate to (2-amino-7-hydroxy-4,6-dioxo-4,5,5a,6,7,8,9a,10-octahydro-3H-pyrano[3,2-g]pteridin-8-yl)methyl triphosphate which is oxidized to 2-amino-6-(2,5-dihydroxy-2-oxido-1,3,2-dioxaphosphinane-4-carbonyl)pteridin-4(3H)-one prior to analysis because of the instability of (2-amino-7-hydroxy-4,6-dioxo-4,5,5a,6,7,8,9a,10-octahydro-3H-pyrano[3,2-g]pteridin-8-yl)methyl triphosphate Bacteria

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP
-
Bacteria cyclic pyranopterin phosphate + diphosphate
-
?

Synonyms

Synonyms Comment Organism
MoaA
-
Bacteria
MoaC
-
Bacteria
molybdenum cofactor biosynthetic enzyme
-
Bacteria

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine MoaA is a radical S-adenosylmethionine (SAM) enzyme Bacteria