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Literature summary for 4.2.1.1 extracted from
- Possible roles for His 208 in the active-site region of chloroplast carbonic anhydrase from Pisum sativum (1999), Arch. Biochem. Biophys., 361, 17-24.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) | Pisum sativum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H208A | site-directed mutagenesis, mutant is more easily inactivated by oxidation than the wild-type enzyme | Pisum sativum |
| H208A/C272A | site-directed mutagenesis | Pisum sativum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetazolamide | - |
Pisum sativum |  |
| Cl- | - |
Pisum sativum | |
| SCN- | - |
Pisum sativum | |
| SO42- | - |
Pisum sativum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Pisum sativum | 9507 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | zinc-containing enzyme | Pisum sativum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24200 | - |
SDS-PAGE | Pisum sativum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2CO3 | Pisum sativum | - |
CO2 + H2O | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pisum sativum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pisum sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cotyledon | - |
Pisum sativum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2CO3 | - |
Pisum sativum | CO2 + H2O | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| carbonic anhydrase | - |
Pisum sativum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
CO2 | pH 6.2, 25°C, mutant H208A | Pisum sativum | |
| 0.135 | - |
CO2 | pH 6.2, 25°C, wild-type | Pisum sativum | |
| 0.28 | - |
CO2 | pH 7.3, 25°C, mutant H208A | Pisum sativum | |
| 0.31 | - |
CO2 | pH 7.3, 25°C, wild-type | Pisum sativum | |
| 0.74 | - |
CO2 | pH 8.8, 25°C, wild-type | Pisum sativum | |
| 1.36 | - |
CO2 | pH 8.8, 25°C, mutant H208A | Pisum sativum | |
| 6.08 | - |
CO2 | pH 8.8, 25°C, mutant H208A | Pisum sativum | |
| 6.08 | - |
CO2 | pH 8.8, 25°C, wild-type | Pisum sativum | |
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