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Literature summary for 4.2.1.1 extracted from

  • Smith, K.S.; Ferry, J.G.
    A plant-type (beta-class) carbonic anhydrase in the thermophilic methanoarchaeon Methanobacterium thermoautotrophicum (1999), J. Bacteriol., 181, 6247-6253.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
DELTAH cab gene hyperexpressed in Escherichia coli Methanothermobacter thermautotrophicus

Inhibitors

Inhibitors Comment Organism Structure
azide
-
Methanothermobacter thermautotrophicus
Iodide
-
Methanothermobacter thermautotrophicus
nitrate
-
Methanothermobacter thermautotrophicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.9
-
CO2 pH 8.5, 25°C Methanothermobacter thermautotrophicus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ zinc enzyme Methanothermobacter thermautotrophicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18900
-
4 * 21000, SDS-PAGE, 4 * 18900, calculated on the basis of the amino acid composition deduced from the nucleotide sequence Methanothermobacter thermautotrophicus
21000
-
4 * 21000, SDS-PAGE, 4 * 18900, calculated on the basis of the amino acid composition deduced from the nucleotide sequence Methanothermobacter thermautotrophicus
90000
-
gel filtration Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
H2CO3 Methanothermobacter thermautotrophicus
-
CO2 + H2O
-
r

Organism

Organism UniProt Comment Textmining
Methanothermobacter thermautotrophicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Methanothermobacter thermautotrophicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
specific activity 39.0 U/mg, one unit = (t0-t)/t, where t0 is the time for the uncatalyzed reaction and t is the time of the catalyzed reaction Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2CO3
-
Methanothermobacter thermautotrophicus CO2 + H2O
-
r
additional information no esterase activity detected with p-phenylacetate as the substrate Methanothermobacter thermautotrophicus ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 21000, SDS-PAGE, 4 * 18900, calculated on the basis of the amino acid composition deduced from the nucleotide sequence Methanothermobacter thermautotrophicus

Synonyms

Synonyms Comment Organism
plant-type (beta-class) carbonic anhydrase
-
Methanothermobacter thermautotrophicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
Methanothermobacter thermautotrophicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
75
-
thermostable up to 75°C Methanothermobacter thermautotrophicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
17000
-
CO2 pH 8.5, 25°C Methanothermobacter thermautotrophicus