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Literature summary for 4.2.1.1 extracted from

  • Bageshwar, U.K.; Premkumar, L.; Gokhman, I.; Savchenko, T.; Sussman, J.L.; Zamir, A.
    Natural protein engineering: a uniquely salt-tolerant, but not halophilic, alpha-type carbonic anhydrase from algae proliferating in low- to hyper-saline environments (2004), Protein Eng. Des. Sel., 17, 191-200.
    View publication on PubMed
Search for more literature for 4.2.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Dunaliella salina

Inhibitors

Inhibitors Comment Organism Structure
acetazolamide IC50: 97 nM Dunaliella salina
ClO4- IC50: 2.2 mM Dunaliella salina
I- IC50: 100 mM Dunaliella salina
N3- IC50: about 50 mM Dunaliella salina
p-aminomethylbenzenesulfonamide IC50: 0.0058 mM Dunaliella salina

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
37.1
-
 H2CO3 pH 6.8, 0.1 M NaCl Dunaliella salina
37.3
-
 H2CO3 pH 6.8, in absence of NaCl Dunaliella salina
45
-
 H2CO3 pH 6.8, 0.5 M NaCl Dunaliella salina

Localization

Localization Comment Organism GeneOntology No. Textmining
cell surface
-
 Dunaliella salina 9986
-

Metals/Ions

Metals/Ions Comment Organism Structure
KCl enzyme retains activity at salt concentrations ranging from low salt to at least 1.5 M NaCl or KCl for CO2 hydration, 2.0 M NaCl for esterase activity and 0.5 M for bicarbonate dehydration Dunaliella salina
NaCl enzyme retains activity at salt concentrations ranging from low salt to at least 1.5 M NaCl or KCl for CO2 hydration, 2.0 M NaCl for esterase activity and 0.5 M for bicarbonate dehydration Dunaliella salina
Zn Zn metalloenzyme Dunaliella salina

Organism

Organism UniProt Comment Textmining
Dunaliella salina P54212
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Dunaliella salina

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2CO3
-
 Dunaliella salina CO2 + H2O
-
 r

Synonyms

Synonyms Comment Organism
alpha-type carbonic anhydrase
-
 Dunaliella salina
dCA I
-
 Dunaliella salina

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
39400
-
 H2CO3 pH 6.8, in absence of NaCl Dunaliella salina
41600
-
 H2CO3 pH 6.8, 0.1 M NaCl Dunaliella salina
45000
-
 H2CO3 pH 6.8, 0.5 M NaCl Dunaliella salina

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000097
-
 IC50: 97 nM Dunaliella salina acetazolamide
0.0058
-
 IC50: 0.0058 mM Dunaliella salina p-aminomethylbenzenesulfonamide
2.2
-
 IC50: 2.2 mM Dunaliella salina ClO4-
50
-
 IC50: about 50 mM Dunaliella salina N3-
100
-
 IC50: 100 mM Dunaliella salina I-