Cloned (Comment) | Organism |
---|---|
expression of mutant enzymes Y7F, N62L, and N67L in Escherichia coli | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
crystals of the HCA II single-site mutants Y7F, N62L, and N67L are obtained using the hanging-drop vapor diffusion method. All crystals are isomorphous and belong to space group P2(1) with mean unit cell dimensions: a = 42.7 A, b = 41.6 A, c = 72.9 A and beta = 104.6° | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
N67L | turnover-number for hydration is 1.4fold lower than wild-type value. Turnover-number for dehydration is 5.8fold lower than wild-type value | Homo sapiens |
Y7F | turnover-number for hydration is identical to wild-type value. Turnover-number for dehydration is 5.8fold lower than wild-type value | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P00918 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CO2 + H2O | roles for residues 7, 62, and 67 in finetuning the properties of His64 for optimal proton transfer in catalysis | Homo sapiens | H2CO3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
carbonic anhydrase II | - |
Homo sapiens |
HCA II | - |
Homo sapiens |