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Literature summary for 4.2.1.1 extracted from
- Purification and some properties of carbonic anhydrase from Elephas trogontherii (Steppe elephant) bone (2007), Indian J. Biochem. Biophys., 44, 252-256.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetazolamide | cytosolic isoenzyme; inner peripheral isoenzyme; integral isoenzyme; outer peripheral isoenzyme | Elephas trogontherii |  |
| KSCN | cytosolic isoenzyme; inner peripheral isoenzyme; integral isoenzyme; outer peripheral isoenzyme | Elephas trogontherii | |
| NaN3 | cytosolic isoenzyme; inner peripheral isoenzyme; integral isoenzyme; outer peripheral isoenzyme | Elephas trogontherii | |
| sulfanilamide | integral isoenzyme; outer peripheral isoenzyme | Elephas trogontherii | |
| sulphanilamide | cytosolic isoenzyme; inner peripheral isoenzyme | Elephas trogontherii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.86 | - |
p-nitrophenyl acetate | integral isoenzyme | Elephas trogontherii | |
| 4.525 | - |
p-nitrophenyl acetate | inner peripheral isoenzyme | Elephas trogontherii | |
| 4.83 | - |
p-nitrophenyl acetate | outer peripheral isoenzyme | Elephas trogontherii | |
| 6.8 | - |
p-nitrophenyl acetate | cytosolic isoenzyme | Elephas trogontherii | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Elephas trogontherii | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
inner peripheral isoenzyme, gel filtration | Elephas trogontherii |
| 36000 | - |
cytosolic isoenzyme, gel filtration | Elephas trogontherii |
| 37000 | - |
outer peripheral isoenzyme, gel filtration | Elephas trogontherii |
| 39000 | - |
integral isoenzyme, gel filtration | Elephas trogontherii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Elephas trogontherii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| cytosolic isoenzyme | Elephas trogontherii |
| inner peripheral isoenzyme | Elephas trogontherii |
| integral isoenzyme | Elephas trogontherii |
| outer peripheral isoenzyme | Elephas trogontherii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| bone | cytosolic peripheral isoenzyme | Elephas trogontherii | - |
| bone | inner peripheral isoenzyme | Elephas trogontherii | - |
| bone | integral peripheral isoenzyme | Elephas trogontherii | - |
| bone | outer peripheral isoenzyme | Elephas trogontherii | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl acetate + H2O | - |
Elephas trogontherii | 4-nitrophenol + acetate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 20 | outer peripheral isoenzyme | Elephas trogontherii |
| 30 | - |
cytosolic isoenzyme | Elephas trogontherii |
| 30 | - |
inner peripheral isoenzyme | Elephas trogontherii |
| 60 | - |
integral isoenzyme | Elephas trogontherii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
integral isoenzyme | Elephas trogontherii |
| 7.5 | 10 | cytosolic isoenzyme | Elephas trogontherii |
| 7.5 | 10 | inner peripheral isoenzyme | Elephas trogontherii |
| 7.5 | 11 | outer peripheral isoenzyme | Elephas trogontherii |
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