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Literature summary for 4.2.1.1 extracted from
- Regulation of expression and biochemical characterization of a beta-class carbonic anhydrase from the plant growth-promoting rhizobacterium, Azospirillum brasilense Sp7 (2009), FEMS Microbiol. Lett., 299, 149-158.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Azospirillum brasilense |
General Stability
| General Stability | Organism |
|---|---|
| the purified protein is unstable in the absence of glycerol and aggregates irreversibly | Azospirillum brasilense |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetazolamide | - |
Azospirillum brasilense |  |
| azide | - |
Azospirillum brasilense | |
| Iodide | - |
Azospirillum brasilense | |
| nitrate | - |
Azospirillum brasilense | |
| sulfonamide | - |
Azospirillum brasilense | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
4 * 29000, SDS-PAGE, the native recombinant enzyme is a homotetramer | Azospirillum brasilense |
| 120000 | - |
apparent molecular mass of the purified protein, gel filtration | Azospirillum brasilense |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Azospirillum brasilense | - |
- |
- |
| Azospirillum brasilense Sp7 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA resin column chromatography | Azospirillum brasilense |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CO2 + H2O | - |
Azospirillum brasilense | H2CO3 | - |
r | |
| CO2 + H2O | - |
Azospirillum brasilense Sp7 | H2CO3 | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 29000, SDS-PAGE, the native recombinant enzyme is a homotetramer | Azospirillum brasilense |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-class carbonic anhydrase | - |
Azospirillum brasilense |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.008 | - |
- |
Azospirillum brasilense | acetazolamide | |
| 0.014 | - |
- |
Azospirillum brasilense | azide | |
| 0.073 | - |
- |
Azospirillum brasilense | nitrate | |
| 0.15 | - |
- |
Azospirillum brasilense | Iodide | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | beta carbonic anhydrase is required for scavenging CO2 from the ambient air | Azospirillum brasilense |
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