Cloned (Comment) | Organism |
---|---|
isozyme LjCAA1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, expression analysis by quantitative real-time RT-PCR, and spatial expression profiling, recombinant expression of His-tagged isozyme in Escherichia coli strain BL21(DE3) | Lotus japonicus |
isozyme LjCAA2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, expression analysis by quantitative real-time RT-PCR, and spatial expression profiling, recombinant expression of His-tagged isozymes in Escherichia coli strain BL21(DE3) | Lotus japonicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics, overview | Lotus japonicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | required, binding structure modelling, overview | Lotus japonicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lotus japonicus | A7DX12 | isozyme LjCAA1; isozymes LjCAA1 and LjCAA2 | - |
Lotus japonicus | A7DX13 | isozyme LjCAA2; isozymes LjCAA1 and LjCAA2 | - |
Lotus japonicus Gifu B-129 | A7DX12 | isozyme LjCAA1; isozymes LjCAA1 and LjCAA2 | - |
Lotus japonicus Gifu B-129 | A7DX13 | isozyme LjCAA2; isozymes LjCAA1 and LjCAA2 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged isozyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Lotus japonicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | expression patterns of isozymes LjCAA1 and LjCAA2 by in situ hybridization, both are expressed in nodule inner cortical cells, vascular bundles and central tissue, overview | Lotus japonicus | - |
root nodule | inner cortical cells, both genes encoding LjCAA1 and LjCAA2 code for nodule enhanced carbonic anhydrase isoforms, which are induced early during nodule development, overview | Lotus japonicus | - |
Subunits | Comment | Organism |
---|---|---|
? | x * 30294.62, isozyme LjCAA1, sequence calculation | Lotus japonicus |
? | x * 30740.77, isozyme LjCAA2, sequence calculation | Lotus japonicus |
More | molecular modeling, overview | Lotus japonicus |
Synonyms | Comment | Organism |
---|---|---|
alpha-type carbonic anhydrase | - |
Lotus japonicus |
CAA1 | - |
Lotus japonicus |
CAA2 | - |
Lotus japonicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
assay at | Lotus japonicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.3 | - |
bicarbonate dehydration assay at | Lotus japonicus |
7.5 | - |
CO2 hydration assay at | Lotus japonicus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Lotus japonicus | isozyme LjCAA2, sequence calculation | - |
6.63 |
Lotus japonicus | isozyme LjCAA1, sequence calculation | - |
9.05 |
Organism | Comment | Expression |
---|---|---|
Lotus japonicus | the gene encoding isozyme LjCAA1 is slightly to moderately down-regulated in ineffective nodules formed by mutant Mesorhizobium loti strains | down |
Lotus japonicus | the gene encoding isozyme LjCAA2 is slightly to moderately down-regulated in ineffective nodules formed by mutant Mesorhizobium loti strains | down |
General Information | Comment | Organism |
---|---|---|
physiological function | isozyme LjCAA1 is involved in nitrogen ficxation/assimilation, but may also be involved in biochemical and physiological processes not directly linked to nitrogen fixation/assimilation | Lotus japonicus |
physiological function | isozyme LjCAA2 is involved in nitrogen fixation/assimilation, but may also be involved in biochemical and physiological processes not directly linked to nitrogen fixation/assimilation | Lotus japonicus |