Literature summary for 4.2.1.1 extracted from

Fasseas, M.K.; Tsikou, D.; Flemetakis, E.; Katinakis, P.
Molecular and biochemical analysis of the alpha class carbonic anhydrases in Caenorhabditis elegans (2011), Mol. Biol. Rep., 38, 1777-1785.

Cloned(Commentary)

Cloned (Comment)	Organism
genes cah-1, cah-2, cah-3, cah-4, cah-5, and cah-6, real-time RT-PCR expression analysis, overview. Gene cah-3 is able to complement the function of Saccharomyces cerevisiae CA, NCE103, in vivo, expression in Saccharomyces cerevisiae wild-type strain CEN.PK2 and in mutant strain CEN.HE28-1A. Recombinant expression of His-tagged CAH-3, CAH-4a and CAH-5 in Escherichia coli strain BL21(DE3), but in vitro activity is only detectable for CAH-4a	Caenorhabditis elegans

Cloned (Comment)

Organism

genes cah-1, cah-2, cah-3, cah-4, cah-5, and cah-6, real-time RT-PCR expression analysis, overview. Gene cah-3 is able to complement the function of Saccharomyces cerevisiae CA, NCE103, in vivo, expression in Saccharomyces cerevisiae wild-type strain CEN.PK2 and in mutant strain CEN.HE28-1A. Recombinant expression of His-tagged CAH-3, CAH-4a and CAH-5 in Escherichia coli strain BL21(DE3), but in vitro activity is only detectable for CAH-4a

Caenorhabditis elegans

Protein Variants

Protein Variants	Comment	Organism
additional information	RNAi by feeding is performed on wild-type worms for all genes, silencing of genes cah-3 and cah-4 reduces the life-span of the worms at 22°C, phenotypes, overview	Caenorhabditis elegans

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	required, isozymes CAH-3, CAH-4 and CAH-5 contain the three zinc-binding histidines that are important for enzyme activity, His94, His96, and His119, and also two ligands that provide a hydrogen bond to stabilize the zinc ion during catalysis, Gln-92 and Glu-117	Caenorhabditis elegans

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
H2CO3	Caenorhabditis elegans	-	CO2 + H2O	-	r

Organism

Organism	UniProt	Comment	Textmining
Caenorhabditis elegans	-	six genes, cah-1, cah-2, cah-3, cah-4, cah-5, and cah-6, possibly encoding alpha class carbonic anhydrases	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant isozymes CAH-3, CAH-4a and CAH-5 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography	Caenorhabditis elegans

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	real-time RT-PCR analysis reveals the temporal expression pattern of genes cah-1, cah-2, cah-3, cah-4, cah-5, and cah-6	Caenorhabditis elegans	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
H2CO3	-	Caenorhabditis elegans	CO2 + H2O	-	r

Synonyms

Synonyms	Comment	Organism
alpha class carbonic anhydrase	-	Caenorhabditis elegans

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
4	-	assay at	Caenorhabditis elegans

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
685000	-	CO2	purified recombinant CAH-4a, pH 7.5, 4°C	Caenorhabditis elegans

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Caenorhabditis elegans

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
66.8	-	CO2	purified recombinant CAH-4a, pH 7.5, 4°C	Caenorhabditis elegans