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Literature summary for 4.2.1.1 extracted from

  • Tripp, B.C.; Bell, C.B.; Cruz, F.; Krebs, C.; Ferry, J.G.
    A role for iron in an ancient carbonic anhydrase (2004), J. Biol. Chem., 279, 6683-6687.
    View publication on PubMed
Search for more literature for 4.2.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Methanosarcina thermophila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic constants of apoenzyme reconstituted with Zn2+, Co2+, Cu2+, Mn2+, Ni2+, Cd2+, or Fe2+ Methanosarcina thermophila

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ anaerobic reconstitution of apoenzyme with Fe2+, which yields an enzyme with an effective kcat that exceeds that for the Zn2+-reconstituted enzyme. The Fe2+-reconstituted enzyme contains high spin Fe2+ that, when oxidized to Fe3+, inactivates the enzyme. Reconstitution with Fe3+ is unsuccessful Methanosarcina thermophila
Fe2+ the Fe2+-reconstituted enzyme produced in Escherichia coli and purified anaerobically contains iron with effective kcat and kcat/Km values exceeding the values for Zn2+-reconstituted enzyme. The Fe2+-reconstituted enzyme contains high spin Fe2+ that, when oxidized to Fe3+, inactivates the enzyme. Reconstitution with Fe3+ is unsuccessful Methanosarcina thermophila
additional information reconstitution of the apoenzyme with Cu2+, Mn2+, Ni2+, or Cd2+ yields enzymes with effective kcat values that are 10% or less than the value for the Zn2+-reconstituted apoenzyme Methanosarcina thermophila
Zn2+ the enzyme contains contains catalytic zinc Methanosarcina thermophila

Organism

Organism UniProt Comment Textmining
Methanosarcina thermophila
-
-
-
Methanosarcina thermophila P40881
-
-

Purification (Commentary)

Purification (Comment) Organism
the recombinant enzyme is purified anaerobically Methanosarcina thermophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CO2 + H2O
-
 Methanosarcina thermophila H2CO3
-
 ?

Synonyms

Synonyms Comment Organism
Cam
-
 Methanosarcina thermophila

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information kinetic constants of apoenzyme reconstituted with Zn2+, Co2+, Cu2+, Mn2+, Ni2+, Cd2+, or Fe2+ Methanosarcina thermophila
additional information
-
 additional information the Fe2+-reconstituted enzyme produced in Escherichia coli and purified anaerobically contains iron with effective kcat and kcat/Km values exceeding the values for Zn2+-reconstituted enzyme Methanosarcina thermophila

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
additional information
-
 additional information kinetic constants of apoenzyme reconstituted with Zn2+, Co2+, Cu2+, Mn2+, Ni2+, Cd2+, or Fe2+ Methanosarcina thermophila
additional information
-
 additional information the Fe2+-reconstituted enzyme produced in Escherichia coli and purified anaerobically contains iron with effective kcat and kcat/Km values exceeding the values for Zn2+-reconstituted enzyme Methanosarcina thermophila