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Literature summary for 4.2.1.1 extracted from

  • Fisher, Z.; Boone, C.D.; Biswas, S.M.; Venkatakrishnan, B.; Aggarwal, M.; Tu, C.; Agbandje-McKenna, M.; Silverman, D.; McKenna, R.
    Kinetic and structural characterization of thermostabilized mutants of human carbonic anhydrase II (2012), Protein Eng. Des. Sel., 25, 347-355.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
all variants crystallized in the orthorhombic P2(1) 2(1) 2 (1) space group and are highly isomorphous with approximate unit cell dimensions a=42, b=72 and c=75 A and diffract between 1.56 and 2.0 A resolution Homo sapiens

Protein Variants

Protein Variants Comment Organism
L100H/L224S/L240P kcat/Km (CO2) decreased compared to wild-type, T(M): 65°C compared to wild-type: 58°C, T (inactivation): 65°C compared to wild-type: 60°C Homo sapiens
L100H/L224S/L240P/Y7F kcat/Km (CO2) slightly decreased compared to wild-type, T(M): 61.1°C compared to wild-type: 58°C, T (inactivation): 65°C compared to wild-type: 60°C Homo sapiens
L100H/L224S/L240P/Y7F/N62L kcat/Km (CO2) decreased compared to wild-type, T(M): 58.1°C compared to wild-type: 58°C, T (inactivation): 60°C compared to wild-type: 60°C Homo sapiens
L100H/L224S/L240P/Y7F/N62L/N67Q kcat/Km (CO2) slightly decreased compared to wild-type, T(M): 59.2°C compared to wild-type: 58°C, T (inactivation): 60°C compared to wild-type: 60°C Homo sapiens
L100H/L224S/L240P/Y7F/N67Q kcat/Km (CO2) decreased compared to wild-type, T(M): 62.7°C compared to wild-type: 58°C, T (inactivation): 65°C compared to wild-type: 60°C Homo sapiens
Y7F kcat/Km (CO2) equal to wild-type, T(M): 53°C compared to wild-type: 58°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P00918
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CO2 + H2O
-
Homo sapiens H2CO3
-
?

Synonyms

Synonyms Comment Organism
hCAII
-
Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
58
-
T(M), wild-type Homo sapiens
60
-
inactivation temperature wild-type Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
85000
-
CO2 mutant L100H/L224S/L240P, pH 7.6, 25°C Homo sapiens
88000
-
CO2 mutant L100H/L224S/L240P/Y7F/N62L, pH 7.6, 25°C Homo sapiens
94000
-
CO2 mutant L100H/L224S/L240P/Y7F/N67Q, pH 7.6, 25°C Homo sapiens
110000
-
CO2 mutant L100H/L224S/L240P/Y7F, pH 7.6, 25°C Homo sapiens
110000
-
CO2 mutant L100H/L224S/L240P/Y7F/N62L/N67Q, pH 7.6, 25°C Homo sapiens
120000
-
CO2 mutant Y7F, pH 7.6, 25°C Homo sapiens
120000
-
CO2 wild-type, pH 7.6, 25°C Homo sapiens