Cloned (Comment) | Organism |
---|---|
gene CA5A, localized to chromosome 16q24.3, an unprocessed pseudogene is assigned to 16p12, recombinant His-tagged enzyme expression in Escherichia coli strain BL21(DE3) from plasmid pENTR223 as soluble enzyme and in inclusion bodies | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2CO3 | Homo sapiens | - |
CO2 + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P35218 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant soluble His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography from the supernatant, and recombinant renatured His-tagged enzyme from inclusion bodies by nickel affinity chromatography, both enzyme forms are active | Homo sapiens |
Renatured (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli inclusion bodies by solubilization of the purified inclusion bodies in 50 mM phosphate buffer, pH 7.0, 300 mM NaCl, and 1% N-lauroylsarcosine, and incubation on rocker for 30 min, or by dialyzing the solution against 50 mM phosphate buffer, pH 7.0, and 150 mM NaCl for 24 h at 4°C, and then followed by centrifugation of the solubilized inclusion bodies for 30 min at 12000 rpm | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2CO3 | - |
Homo sapiens | CO2 + H2O | - |
r | |
additional information | 4-nitrophenyl acetate is used as the substrate to measure the activity of enzyme CAVA | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30000, recombinant enzyme incuding signal peptide, SDS-PAGE | Homo sapiens |
More | peptide mass finger prints by tryptic digestion and mass spectrometry. Secondary structure of enzyme CAVA is measured using circular dichroism. The secondary structural contents for the native CAVA include 20.2% alpha-helix, 29.1% beta-sheet and 50.7% random coil, while the renatured CAVA has 21.7% alpha-helix, 27.2% beta-sheet, and 51.3% random coil, and the denatured CAVA has 15.3% alpha-helix, 21.7% beta-sheet, and 63% random coil | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
CA5A | - |
Homo sapiens |
carbonic anhydrase 5A | - |
Homo sapiens |
carbonic anhydrase VA | - |
Homo sapiens |
CAVA | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | carbonic anhydrase VA (CAVA) is a mitochondrial enzyme that catalyzes the reversible hydration of CO2 to produce HCO3- and proton. CAVA is primarily involved in several biosynthetic processes such as ureagenesis, gluconeogenesis and lipogenesis by providing bicarbonate ion | Homo sapiens |