Literature summary for 4.2.1.1 extracted from

Idrees, D.; Kumar, S.; Rehman, S.A.A.; Gourinath, S.; Islam, A.; Ahmad, F.; Imtaiyaz Hassan, M.
Cloning, expression, purification and characterization of human mitochondrial carbonic anhydrase VA (2016), 3 Biotech, 6, 16 .

Cloned(Commentary)

Cloned (Comment)	Organism
gene CA5A, localized to chromosome 16q24.3, an unprocessed pseudogene is assigned to 16p12, recombinant His-tagged enzyme expression in Escherichia coli strain BL21(DE3) from plasmid pENTR223 as soluble enzyme and in inclusion bodies	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	-	Homo sapiens	5739	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
H2CO3	Homo sapiens	-	CO2 + H2O	-	r

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P35218	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant soluble His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography from the supernatant, and recombinant renatured His-tagged enzyme from inclusion bodies by nickel affinity chromatography, both enzyme forms are active	Homo sapiens

Renatured (Commentary)

Renatured (Comment)	Organism
recombinant enzyme from Escherichia coli inclusion bodies by solubilization of the purified inclusion bodies in 50 mM phosphate buffer, pH 7.0, 300 mM NaCl, and 1% N-lauroylsarcosine, and incubation on rocker for 30 min, or by dialyzing the solution against 50 mM phosphate buffer, pH 7.0, and 150 mM NaCl for 24 h at 4°C, and then followed by centrifugation of the solubilized inclusion bodies for 30 min at 12000 rpm	Homo sapiens

Renatured (Comment)

Organism

recombinant enzyme from Escherichia coli inclusion bodies by solubilization of the purified inclusion bodies in 50 mM phosphate buffer, pH 7.0, 300 mM NaCl, and 1% N-lauroylsarcosine, and incubation on rocker for 30 min, or by dialyzing the solution against 50 mM phosphate buffer, pH 7.0, and 150 mM NaCl for 24 h at 4°C, and then followed by centrifugation of the solubilized inclusion bodies for 30 min at 12000 rpm

Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
H2CO3	-	Homo sapiens	CO2 + H2O	-	r
additional information	4-nitrophenyl acetate is used as the substrate to measure the activity of enzyme CAVA	Homo sapiens	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 30000, recombinant enzyme incuding signal peptide, SDS-PAGE	Homo sapiens
More	peptide mass finger prints by tryptic digestion and mass spectrometry. Secondary structure of enzyme CAVA is measured using circular dichroism. The secondary structural contents for the native CAVA include 20.2% alpha-helix, 29.1% beta-sheet and 50.7% random coil, while the renatured CAVA has 21.7% alpha-helix, 27.2% beta-sheet, and 51.3% random coil, and the denatured CAVA has 15.3% alpha-helix, 21.7% beta-sheet, and 63% random coil	Homo sapiens

Synonyms

Synonyms	Comment	Organism
CA5A	-	Homo sapiens
carbonic anhydrase 5A	-	Homo sapiens
carbonic anhydrase VA	-	Homo sapiens
CAVA	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Homo sapiens

General Information

General Information	Comment	Organism
physiological function	carbonic anhydrase VA (CAVA) is a mitochondrial enzyme that catalyzes the reversible hydration of CO2 to produce HCO3- and proton. CAVA is primarily involved in several biosynthetic processes such as ureagenesis, gluconeogenesis and lipogenesis by providing bicarbonate ion	Homo sapiens