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Literature summary for 4.2.1.1 extracted from

  • De Luca, V.; Vullo, D.; Del Prete, S.; Carginale, V.; Osman, S.M.; AlOthman, Z.; Supuran, C.T.; Capasso, C.
    Cloning, characterization and anion inhibition studies of a gamma-carbonic anhydrase from the Antarctic bacterium Colwellia psychrerythraea (2016), Bioorg. Med. Chem., 24, 835-840 .
    View publication on PubMed
Search for more literature for 4.2.1.1

Cloned(Commentary)

Cloned (Comment) Organism
gene CpsCAgamma, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) (Arctic Express DE3 competent cells), subcloning in Escherichia coli strain DH5alpha Colwellia psychrerythraea

Inhibitors

Inhibitors Comment Organism Structure
acetazolamide sulfonamide CA inhibitor (CAI) Colwellia psychrerythraea
B4O72-
-
 Colwellia psychrerythraea
CF3SO3-
-
 Colwellia psychrerythraea
Cl-
-
 Colwellia psychrerythraea
CN-
-
 Colwellia psychrerythraea
CNO-
-
 Colwellia psychrerythraea
CO32-
-
 Colwellia psychrerythraea
CS32-
-
 Colwellia psychrerythraea
Et2NCS2-
-
 Colwellia psychrerythraea
FSO3-
-
 Colwellia psychrerythraea
H2NSO2NH2
-
 Colwellia psychrerythraea
H2NSO3H
-
 Colwellia psychrerythraea
HCO3-
-
 Colwellia psychrerythraea
HS-
-
 Colwellia psychrerythraea
HSO3-
-
 Colwellia psychrerythraea
I-
-
 Colwellia psychrerythraea
additional information perchlorate, tetrafluoroborate, azide, fluoride, and bromide are not inhibitory for the enzyme Colwellia psychrerythraea
NH(SO3)22-
-
 Colwellia psychrerythraea
NO2-
-
 Colwellia psychrerythraea
NO3-
-
 Colwellia psychrerythraea
P2O74-
-
 Colwellia psychrerythraea
PF6-
-
 Colwellia psychrerythraea
Ph-AsO3H2
-
 Colwellia psychrerythraea
Ph-B(OH)2
-
 Colwellia psychrerythraea
ReO4-
-
 Colwellia psychrerythraea
RuO4-
-
 Colwellia psychrerythraea
S2O82-
-
 Colwellia psychrerythraea
SCN-
-
 Colwellia psychrerythraea
SeCN-
-
 Colwellia psychrerythraea
SeO42-
-
 Colwellia psychrerythraea
SnO32-
-
 Colwellia psychrerythraea
SO42-
-
 Colwellia psychrerythraea
TeO42-
-
 Colwellia psychrerythraea
V2O74-
-
 Colwellia psychrerythraea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4700
-
 CO2 pH 8.3, 22°C Colwellia psychrerythraea

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ required, metalloenzyme. The three amino acid residues Asn73, Gln75 and Asn202, which constitute the triad involved in the network of hydrogen bonds with the zinc-coordinated water molecule, are conserved in all the sequences of gamma-CA enzymes. The enzyme CpsCA trimer contains 3 active sites and each monomer contributes His residues located on the surface to coordinate the Zn(II) ion crucial for catalysis Colwellia psychrerythraea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 recombinant enzyme, native PAGE Colwellia psychrerythraea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CO2 + H2O Colwellia psychrerythraea
-
 H2CO3
-
 r
CO2 + H2O Colwellia psychrerythraea 34H / ATCC BAA-681
-
 H2CO3
-
 r
H2CO3 Colwellia psychrerythraea
-
 CO2 + H2O
-
 r
H2CO3 Colwellia psychrerythraea 34H / ATCC BAA-681
-
 CO2 + H2O
-
 r
additional information Colwellia psychrerythraea the enzyme shows a significant catalytic activity for the physiologic reaction of CO2 hydration to bicarbonate and protons ?
-
 ?
additional information Colwellia psychrerythraea 34H / ATCC BAA-681 the enzyme shows a significant catalytic activity for the physiologic reaction of CO2 hydration to bicarbonate and protons ?
-
 ?

Organism

Organism UniProt Comment Textmining
Colwellia psychrerythraea Q47YG3 i.e. Vibrio psychroerythus
-
Colwellia psychrerythraea 34H / ATCC BAA-681 Q47YG3 i.e. Vibrio psychroerythus
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)/ArcticExpress DE3 cells by nickel affinity chromatography Colwellia psychrerythraea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CO2 + H2O
-
 Colwellia psychrerythraea H2CO3
-
 r
CO2 + H2O
-
 Colwellia psychrerythraea 34H / ATCC BAA-681 H2CO3
-
 r
H2CO3
-
 Colwellia psychrerythraea CO2 + H2O
-
 r
H2CO3
-
 Colwellia psychrerythraea 34H / ATCC BAA-681 CO2 + H2O
-
 r
additional information the enzyme shows a significant catalytic activity for the physiologic reaction of CO2 hydration to bicarbonate and protons Colwellia psychrerythraea ?
-
 ?
additional information the enzyme shows a significant catalytic activity for the physiologic reaction of CO2 hydration to bicarbonate and protons Colwellia psychrerythraea 34H / ATCC BAA-681 ?
-
 ?

Subunits

Subunits Comment Organism
homotrimer 3 * 20000, recombinant enzyme, SDS-PAGE Colwellia psychrerythraea
More cross-sectional profiles of the gamma-CA trimer reveal that each monomer resembles an equilateral triangle, which is stabilized by hydrogen bonds, salt bridges and hydrophobic interactions. The trimer contains 3 active sites and each monomer contributes His residues located on the surface to coordinate the Zn(II) ion crucial for catalysis Colwellia psychrerythraea

Synonyms

Synonyms Comment Organism
CpsCA
-
 Colwellia psychrerythraea
CpsCAgamma
-
 Colwellia psychrerythraea
gamma-CA
-
 Colwellia psychrerythraea
gamma-carbonic anhydrase
-
 Colwellia psychrerythraea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperature Colwellia psychrerythraea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
600000
-
 CO2 pH 8.3, 22°C Colwellia psychrerythraea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3
-
 assay at Colwellia psychrerythraea

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000502
-
 acetazolamide pH 8.3, 22°C Colwellia psychrerythraea
0.008
-
 H2NSO2NH2 pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
0.026
-
 Ph-B(OH)2 pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
0.037
-
 H2NSO3H pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
0.038
-
 Ph-AsO3H2 pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
0.58
-
 Et2NCS2- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
1.4
-
 CO32- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
1.7
-
 HCO3- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
1.8
-
 SnO32- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
2.5
-
 CNO- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
3.3
-
 SCN- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
3.8
-
 CS32- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
4.3
-
 FSO3- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
4.4
-
 CN- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
4.5
-
 S2O82- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
4.7
-
 B4O72- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
4.8
-
 HS- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
5.1
-
 TeO42- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
5.6
-
 PF6- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
5.8
-
 V2O74- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
6
-
 SeCN- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
7.3
-
 P2O74- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
7.7
-
 CF3SO3- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
7.8
-
 ReO4- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
7.9
-
 RuO4- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
7.9
-
 NH(SO3)22- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
8.3
-
 SeO42- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
8.7
-
 I- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
8.8
-
 NO2- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
9
-
 Cl- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
9.4
-
 SO42- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
21.7
-
 NO3- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
37.5
-
 HSO3- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea