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Literature summary for 4.2.1.1 extracted from

  • Esbaugh, A.J.; Secor, S.M.; Grosell, M.
    Characterization of carbonic anhydrase XIII in the erythrocytes of the Burmese python, Python molurus bivittatus (2015), Comp. Biochem. Physiol. B, 187, 71-77 .
    View publication on PubMed
Search for more literature for 4.2.1.1

Cloned(Commentary)

Cloned (Comment) Organism
cloning of isozyme CA XIII, phylogenetic analysis, quantitative real-time PCR expression analysis Python bivittatus

Inhibitors

Inhibitors Comment Organism Structure
azetazolamide
-
 Python bivittatus
CNO-
-
 Python bivittatus
Cu2+
-
 Python bivittatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
21.5
-
 H2CO3 pH not specified in the publication, 4°C, assay using the delta pH method Python bivittatus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Python bivittatus 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ required, metalloenzyme Python bivittatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
H2CO3 Python bivittatus
-
 CO2 + H2O
-
 r

Organism

Organism UniProt Comment Textmining
Python bivittatus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte about 90% of the CA activity in erythrocytes is due to the high activity CA II-like isoform, 10% are due to a low activity CA XIII isozyme Python bivittatus
-
erythrocyte erythrocyte isoform CA XIII. About 90% of the CA activity in erythrocytes is due to a high activity CA II-like isoform, 10% are due to the low activity CA XIII isozyme Python bivittatus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2CO3
-
 Python bivittatus CO2 + H2O
-
 r
additional information assay using the delta pH method at 4°C Python bivittatus ?
-
 ?

Synonyms

Synonyms Comment Organism
alpha-carbonic anhydrase
-
 Python bivittatus
carbonic anhydrase II
-
 Python bivittatus
carbonic anhydrase XIII
-
 Python bivittatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
13000
-
 H2CO3 pH not specified in the publication, 4°C, assay using the delta pH method Python bivittatus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00000064
-
 azetazolamide pH not specified in the publication, 4°C, assay using the delta pH method Python bivittatus
0.0004
-
 Cu2+ pH not specified in the publication, 4°C, assay using the delta pH method Python bivittatus
0.0024
-
 CNO- pH not specified in the publication, 4°C, assay using the delta pH method Python bivittatus
0.0309
-
 Cu2+ pH not specified in the publication, 4°C, assay using the delta pH method Python bivittatus

Expression

Organism Comment Expression
Python bivittatus feeding stress is used to upregulate CO2 excretion pathways: significant upregulation of isozyme CA II is observed, while isozyme CA XIII is strongly downregulated down

General Information

General Information Comment Organism
physiological function carbonic anhydrase (CA) is a ubiquitously expressed metalloenzyme that catalyses the hydration and dehydration reactions of carbon dioxide and bicarbonate, respectively. This basic reaction has innumerable physiological and biochemical associations and consequently CA isoforms are found in virtually all living organisms Python bivittatus