Application | Comment | Organism |
---|---|---|
additional information | carbonic anhydrase is a biocatalyst that catalyzes the hydration of CO2 to bicarbonate and protons, thus useful in mitigating green house effect by sequestering CO2 from various point sources | Halalkalibacterium halodurans |
Cloned (Comment) | Organism |
---|---|
recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) achieving a 31.4fold enhancement in CA production for the wild-type, subcloning in Escherichia coli strain DH5alpha. About 50% of wild-type enzyme produced is secreted when recombinant Escherichia coli harboring BhCA-pET22b is cultivated in a medium with EDTA and lysozyme because of the efficient pelB leader sequence, cultvation parameters are optimized for rBhCA secretion | Halalkalibacterium halodurans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,6-PDCA | - |
Halalkalibacterium halodurans | |
2-mercaptoethanol | 15% inhibition at 5 mM | Halalkalibacterium halodurans | |
acetazolamide | strong inhibition at 5 mM | Halalkalibacterium halodurans | |
Ag+ | inhibits by 17% at 5 mM, no inhibition at 1 mM | Halalkalibacterium halodurans | |
Al3+ | inhibits by 12% at 5 mM, no inhibition at 1 mM | Halalkalibacterium halodurans | |
Co2+ | inhibits by 74% at 5 mM | Halalkalibacterium halodurans | |
Cu2+ | inhibits completely at 5 mM, 66% inhibition at 1 mM | Halalkalibacterium halodurans | |
diethyl dicarbonate | strong inhibition at 5 mM | Halalkalibacterium halodurans | |
DTT | 60% inhibition at 5 mM | Halalkalibacterium halodurans | |
EDTA | - |
Halalkalibacterium halodurans | |
ethyl 3-(3-dimethylaminopropyl)-carbodiimide | 80% inhibition at 5 mM, via irreversible modification of carboxyl groups or thepresence of catalytically active tyrosine in the enzyme molecule | Halalkalibacterium halodurans | |
Fe2+ | inhibits completely at 5 mM, 74% inhibition at 1 mM | Halalkalibacterium halodurans | |
Fe3+ | inhibits completely at 5 mM, 75% inhibition at 1 mM | Halalkalibacterium halodurans | |
Hg2+ | inhibits completely at 5 mM, 78% inhibition at 1 mM | Halalkalibacterium halodurans | |
iodoacetic acid | - |
Halalkalibacterium halodurans | |
N-bromosuccinimide | strong inhibition at 5 mM | Halalkalibacterium halodurans | |
N-ethylmaleimide | strong inhibition at 5 mM | Halalkalibacterium halodurans | |
Na+ | inhibits by 25% at 2 mM | Halalkalibacterium halodurans | |
Ni2+ | inhibits by 84% at 5 mM, 80% inhibition at 1 mM | Halalkalibacterium halodurans | |
Pb2+ | inhibits by 30% at 5 mM | Halalkalibacterium halodurans | |
PMSF | 80% inhibition at 5 mM | Halalkalibacterium halodurans | |
SDS | - |
Halalkalibacterium halodurans | |
Triton X-100 | - |
Halalkalibacterium halodurans | |
Tween 80 | - |
Halalkalibacterium halodurans | |
Woodward's reagent K | WRK | Halalkalibacterium halodurans | |
Zn2+ | inhibits by 95% at 5 mM | Halalkalibacterium halodurans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | BhCA also functions as an efficient virtual peroxidase when Zn2+ is substituted with Mn2+ | Halalkalibacterium halodurans | |
additional information | no effect on enzyme activity at 5 mM by Ca2+, NH4+, Mg2+, Ba2+, and Mn2+ | Halalkalibacterium halodurans | |
Sn2+ | activates about 40% at 5 mM | Halalkalibacterium halodurans | |
Zn2+ | required, metalloenzyme | Halalkalibacterium halodurans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | - |
about | Halalkalibacterium halodurans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CO2 + H2O | Halalkalibacterium halodurans | - |
H2CO3 | - |
r | |
CO2 + H2O | Halalkalibacterium halodurans TSLV1 | - |
H2CO3 | - |
r | |
H2CO3 | Halalkalibacterium halodurans | - |
CO2 + H2O | - |
r | |
H2CO3 | Halalkalibacterium halodurans TSLV1 | - |
CO2 + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Halalkalibacterium halodurans | A0A0N9WRG3 | - |
- |
Halalkalibacterium halodurans TSLV1 | A0A0N9WRG3 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) medium by nickel affinity chromatography | Halalkalibacterium halodurans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CO2 + H2O | - |
Halalkalibacterium halodurans | H2CO3 | - |
r | |
CO2 + H2O | - |
Halalkalibacterium halodurans TSLV1 | H2CO3 | - |
r | |
H2CO3 | - |
Halalkalibacterium halodurans | CO2 + H2O | - |
r | |
H2CO3 | - |
Halalkalibacterium halodurans TSLV1 | CO2 + H2O | - |
r | |
additional information | mineralization of CO2 using recombinant BhCA leads to accelerated precipitation of CaCO3 in calcite form. Recombinant BhCA also functions as an efficient virtual peroxidase when Zn2+ is substituted with Mn2+ | Halalkalibacterium halodurans | ? | - |
? | |
additional information | mineralization of CO2 using recombinant BhCA leads to accelerated precipitation of CaCO3 in calcite form. Recombinant BhCA also functions as an efficient virtual peroxidase when Zn2+ is substituted with Mn2+ | Halalkalibacterium halodurans TSLV1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Halalkalibacterium halodurans |
More | structural secondary composition of rBhCA at different pH values, overview | Halalkalibacterium halodurans |
Synonyms | Comment | Organism |
---|---|---|
alpha carbonic anhydrase | - |
Halalkalibacterium halodurans |
alpha-carbonic anhydrase | - |
Halalkalibacterium halodurans |
BhCA | - |
Halalkalibacterium halodurans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Halalkalibacterium halodurans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 72 | Tm of BhCA is 72°C, T1/2 values at 50°C and 60°C are 66 min and 24 min, respectively | Halalkalibacterium halodurans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | - |
assay at | Halalkalibacterium halodurans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 11 | purified recombinant enzyme, completely stable within this range for 48 h | Halalkalibacterium halodurans |
General Information | Comment | Organism |
---|---|---|
additional information | residues H137, H139, H156, and H110 present in the active site play an important role in catalysis, the active site of recombinant BhCA is also composed of conserved Glu and Asp acid residues | Halalkalibacterium halodurans |
physiological function | carbonic anhydrase is a biocatalyst that catalyzes the hydration of CO2to bicarbonate and protons, and an alkalistable and moderately thermostable alpha-carbonic anhydrase | Halalkalibacterium halodurans |