Any feedback?
Literature summary for 4.2.1.1 extracted from
- Characterization of folding intermediates during urea-induced denaturation of human carbonic anhydrase II (2017), Int. J. Biol. Macromol., 95, 881-887 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene CA2, recombinant expression of His-tagged isozyme HCAII | Homo sapiens |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | required, metalloenzyme | Homo sapiens |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2CO3 | Homo sapiens | - |
CO2 + H2O | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P00918 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged isozyme HCAII by nickel affinity chromatography, ultrafiltration, and gel filtration, to homogeneity | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2CO3 | - |
Homo sapiens | CO2 + H2O | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 29000, recombinant enzyme, SDS-PAGE | Homo sapiens |
| More | isozyme HCAII is a beta-sheet dominated monomeric protein of 259 aminoacid residues. The far-UV CD is used to characterize the intermediate states (XI and XII) for secondary structural content, near-UV CD for tertiary structure, dynamic light scatteringfor hydrodynamic radius and ANS fluorescence spectroscopy for the presence of exposed hydrophobic patches. Stable intermediates XI and XII populations of intermediates are prepared by urea-derived protein denaturation at around 2 and 4 M urea, respectively. Urea-induced XI state has characteristics ofmolten globule state while XII state bears characteristics features of pre-molten globule state, overview | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CA II | - |
Homo sapiens |
| CA2 | - |
Homo sapiens |
| carbonic anhydrase II | - |
Homo sapiens |
| hCAII | - |
Homo sapiens |
| human carbonic anhydrase II | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
