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Literature summary for 4.2.1.1 extracted from

  • Orhan, F.; Sentuerk, M.; Supuran, C.T.
    Interaction of anions with a newly characterized alpha carbonic anhydrase from Halomonas sp. (2016), J. Enzyme Inhib. Med. Chem., 31, 1119-1123 .
    View publication on PubMed
Search for more literature for 4.2.1.1

Inhibitors

Inhibitors Comment Organism Structure
Br-
-
 Halomonas sp.
Cl-
-
 Halomonas sp.
CO32-
-
 Halomonas sp.
I-
-
 Halomonas sp.
additional information sulfate, sulfide, azide, nitrate, nitrite and iodide exhibit strong inhibitory activity in the micromolar range (Ki values of 5.5-15.5 mM). Cyanide, bromide, chloride, and carbonate are moderate inhibitors, whereas other anions show only weak activities. The anions inhibit enzyme HmCA in a similar manner to other alpha-CAs from mammals, but the susceptibility to various anions differs significantly between the Halomonas sp. and other organism CAs, overview Halomonas sp.
N3-
-
 Halomonas sp.
NO2-
-
 Halomonas sp.
NO3-
-
 Halomonas sp.
SCN-
-
 Halomonas sp.
SO32-
-
 Halomonas sp.
SO42-
-
 Halomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme has an optimum ionic strength at 20 mM. Sulfate, sulfide, azide, nitrate, nitrite and iodide exhibit strong inhibitory activity in the micromolar range (Ki values of 5.5-15.5 mM). Cyanide, bromide, chloride, and carbonate are moderate inhibitors, whereas other anions show only weak activities Halomonas sp.
Zn2+ required, metalloenzyme Halomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
H2CO3 Halomonas sp.
-
 CO2 + H2O
-
 r

Organism

Organism UniProt Comment Textmining
Halomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 91fold by L-tyrosine-sulfanilamide affinity chromatography, elution with 1 M NaCl/25 mM Na2HPO4, pH 6.3 Halomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 600 EU/mg protein, purified native enzyme, pH 7.5, 20°C Halomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CO2 + H2O
-
 Halomonas sp. H2CO3
-
 r
H2CO3
-
 Halomonas sp. CO2 + H2O
-
 r
additional information esterase activity assay with 4-nitrophenyl acetate as substrate and measurement of CO2 hydration reaction for determination of enzyme activity Halomonas sp. ?
-
 ?

Synonyms

Synonyms Comment Organism
alpha carbonic anhydrase
-
 Halomonas sp.
alpha-CA
-
 Halomonas sp.
alpha-class carbonic anhydrase
-
 Halomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
-
 Halomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Halomonas sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0055
-
 SO42- pH 7.5, 20°C, inhibition of CO2 hydration reaction Halomonas sp.
0.008
-
 SO32- pH 7.5, 20°C, inhibition of CO2 hydration reaction Halomonas sp.
0.0085
-
 I- pH 7.5, 20°C, inhibition of CO2 hydration reaction Halomonas sp.
0.01
-
 N3- pH 7.5, 20°C, inhibition of CO2 hydration reaction Halomonas sp.
0.01
-
 NO3- pH 7.5, 20°C, inhibition of CO2 hydration reaction Halomonas sp.
0.0155
-
 NO2- pH 7.5, 20°C, inhibition of CO2 hydration reaction Halomonas sp.
0.0275
-
 Br- pH 7.5, 20°C, inhibition of CO2 hydration reaction Halomonas sp.
0.0285
-
 SCN- pH 7.5, 20°C, inhibition of CO2 hydration reaction Halomonas sp.
0.039
-
 Cl- pH 7.5, 20°C, inhibition of CO2 hydration reaction Halomonas sp.
0.121
-
 CO32- pH 7.5, 20°C, inhibition of CO2 hydration reaction Halomonas sp.