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Literature summary for 4.2.1.1 extracted from

  • Del Prete, S.; De Luca, V.; De Simone, G.; Supuran, C.T.; Capasso, C.
    Cloning, expression and purification of the complete domain of the eta-carbonic anhydrase from Plasmodium falciparum (2016), J. Enzyme Inhib. Med. Chem., 31, 54-59 .
    View publication on PubMed

Application

Application Comment Organism
drug development superfamily of metalloenzymes carbonic anhydrases (CAs) has a potential as druggable enzymes to limit the development of Plasmodium falciparum gametocytes Plasmodium falciparum

Cloned(Commentary)

Cloned (Comment) Organism
enzyme form PfCAdom comprising 358 amino acids (residue 181 to residue 538), recombinant expression of His-tagged enzyme PfCAdom Plasmodium falciparum

Protein Variants

Protein Variants Comment Organism
additional information generation of truncated mutant PfCA1 comprising 231 amino acids, the mutant shows 2.7fold lower kcat and a 13fold lower catalytic efficiency than enzyme PfCAdom Plasmodium falciparum

Inhibitors

Inhibitors Comment Organism Structure
acetazolamide
-
Plasmodium falciparum

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ required for activity, metalloenzyme Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
H2CO3 Plasmodium falciparum
-
CO2 + H2O
-
r

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum Q8IHW5
-
-

Source Tissue

Source Tissue Comment Organism Textmining
gametocyte
-
Plasmodium falciparum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2CO3
-
Plasmodium falciparum CO2 + H2O
-
r

Subunits

Subunits Comment Organism
? x * 42300, about, enzyme PfCAdom, sequence calculation Plasmodium falciparum

Synonyms

Synonyms Comment Organism
eta-carbonic anhydrase
-
Plasmodium falciparum
PF3D7_1140000
-
Plasmodium falciparum
PfCAdom
-
Plasmodium falciparum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at room temperature Plasmodium falciparum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
140000
-
H2CO3 truncated mutant PfCA1, pH 8.3, 22°C Plasmodium falciparum
380000
-
H2CO3 enzyme PfCAdom, pH 8.3, 22°C Plasmodium falciparum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3
-
assay at Plasmodium falciparum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00017
-
acetazolamide truncated mutant PfCA1, pH 8.3, 22°C Plasmodium falciparum
0.000366
-
acetazolamide enzyme PfCAdom, pH 8.3, 22°C Plasmodium falciparum

General Information

General Information Comment Organism
evolution the enzyme belongs to the superfamily of metalloenzymes Plasmodium falciparum
additional information residue Thr477 in PfCAdom is crucial for its catalysis Plasmodium falciparum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5400
-
H2CO3 truncated mutant PfCA1, pH 8.3, 22°C Plasmodium falciparum
72000
-
H2CO3 enzyme PfCAdom, pH 8.3, 22°C Plasmodium falciparum