Application | Comment | Organism |
---|---|---|
drug development | superfamily of metalloenzymes carbonic anhydrases (CAs) has a potential as druggable enzymes to limit the development of Plasmodium falciparum gametocytes | Plasmodium falciparum |
Cloned (Comment) | Organism |
---|---|
enzyme form PfCAdom comprising 358 amino acids (residue 181 to residue 538), recombinant expression of His-tagged enzyme PfCAdom | Plasmodium falciparum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of truncated mutant PfCA1 comprising 231 amino acids, the mutant shows 2.7fold lower kcat and a 13fold lower catalytic efficiency than enzyme PfCAdom | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetazolamide | - |
Plasmodium falciparum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | required for activity, metalloenzyme | Plasmodium falciparum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2CO3 | Plasmodium falciparum | - |
CO2 + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | Q8IHW5 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
gametocyte | - |
Plasmodium falciparum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2CO3 | - |
Plasmodium falciparum | CO2 + H2O | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 42300, about, enzyme PfCAdom, sequence calculation | Plasmodium falciparum |
Synonyms | Comment | Organism |
---|---|---|
eta-carbonic anhydrase | - |
Plasmodium falciparum |
PF3D7_1140000 | - |
Plasmodium falciparum |
PfCAdom | - |
Plasmodium falciparum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Plasmodium falciparum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
140000 | - |
H2CO3 | truncated mutant PfCA1, pH 8.3, 22°C | Plasmodium falciparum | |
380000 | - |
H2CO3 | enzyme PfCAdom, pH 8.3, 22°C | Plasmodium falciparum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | - |
assay at | Plasmodium falciparum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00017 | - |
acetazolamide | truncated mutant PfCA1, pH 8.3, 22°C | Plasmodium falciparum | |
0.000366 | - |
acetazolamide | enzyme PfCAdom, pH 8.3, 22°C | Plasmodium falciparum |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the superfamily of metalloenzymes | Plasmodium falciparum |
additional information | residue Thr477 in PfCAdom is crucial for its catalysis | Plasmodium falciparum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5400 | - |
H2CO3 | truncated mutant PfCA1, pH 8.3, 22°C | Plasmodium falciparum | |
72000 | - |
H2CO3 | enzyme PfCAdom, pH 8.3, 22°C | Plasmodium falciparum |