Any feedback?
Literature summary for 4.2.1.1 extracted from
- Carbonic anhydrase from Apis mellifera purification and inhibition by pesticides (2017), J. Enzyme Inhib. Med. Chem., 32, 47-50 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Atrazine | - |
Apis mellifera |  |
| Carbaryl | - |
Apis mellifera | |
| carbofuran | - |
Apis mellifera | |
| propoxur | - |
Apis mellifera | |
| simazine | - |
Apis mellifera | |
| tebuconazole | - |
Apis mellifera | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | required, metalloenzyme | Apis mellifera | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2CO3 | Apis mellifera | - |
CO2 + H2O | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Apis mellifera | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 35fold from spermatheca by L-tyrosine-sulfanylamide affinity chromatography, elution with 1 M NaCl/25 mM Na2HPO4, pH 6.3 | Apis mellifera |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| spermatheca | - |
Apis mellifera | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
175 EU/mg protein activity in CO2 hydration reaction, purified native enzyme, pH and temperature not specified in the publication | Apis mellifera |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CO2 + H2O | - |
Apis mellifera | H2CO3 | - |
r | |
| H2CO3 | - |
Apis mellifera | CO2 + H2O | - |
r | |
| additional information | the enzyme is also active in an esterase assay with 4-nitrophenylacetate as substrate | Apis mellifera | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 32000, SDS-PAGE | Apis mellifera |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Am CA | - |
Apis mellifera |
| AmCA | - |
Apis mellifera |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| additional information | - |
AmCA inhibition by the pesticides is also measured (IC50 values) using an esterase assay with 4-nitrophenylacetate as substrate | Apis mellifera | additional information | |
| 0.003 | - |
measured with hydration of CO2 activity assay, pH and temperature not specified in the publication | Apis mellifera | tebuconazole | |
| 0.0031 | - |
measured with hydration of CO2 activity assay, pH and temperature not specified in the publication | Apis mellifera | Carbaryl | |
| 0.0087 | - |
measured with hydration of CO2 activity assay, pH and temperature not specified in the publication | Apis mellifera | carbofuran | |
| 0.0165 | - |
measured with hydration of CO2 activity assay, pH and temperature not specified in the publication | Apis mellifera | Atrazine | |
| 0.0273 | - |
measured with hydration of CO2 activity assay, pH and temperature not specified in the publication | Apis mellifera | simazine | |
| 0.0321 | - |
measured with hydration of CO2 activity assay, pH and temperature not specified in the publication | Apis mellifera | propoxur | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
