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Literature summary for 4.2.1.1 extracted from

  • Kanth, B.; Jun, S.; Kumari, S.; Pack, S.
    Highly thermostable carbonic anhydrase from Persephonella marina EX-H1 its expression and characterization for CO2-sequestration applications (2014), Process Biochem., 49, 2114-2121 .
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
gene PERMA_1443, phylogenetic tree, recombinant expression of His-tagged enzyme lacking the signal peptide from codon-optimized carbonic anhydrase gene in Escherichia coli strain BL21(DE3), real-time PCR enzyme expression analysis Persephonella marina

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
Persephonella marina
Al3+
-
Persephonella marina
Cu2+
-
Persephonella marina
Fe3+
-
Persephonella marina
Hg2+
-
Persephonella marina
Pb2+
-
Persephonella marina

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
10.8
-
CO2 pH 7.8, 25°C, recombinant enzyme Persephonella marina

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates the recombinant enzyme lacking the signal peptide Persephonella marina
additional information the recombinant enzyme activity is not affected significantly by the presence of Ca2+, Mg2+, or K+ ions Persephonella marina
Zn2+ required, metalloenzyme, activates the recombinant enzyme lacking the signal peptide Persephonella marina

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
H2CO3 Persephonella marina
-
CO2 + H2O
-
r
H2CO3 Persephonella marina DSM 14350 / EX-H1
-
CO2 + H2O
-
r

Organism

Organism UniProt Comment Textmining
Persephonella marina C0QRB5
-
-
Persephonella marina DSM 14350 / EX-H1 C0QRB5
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration Persephonella marina

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CO2 + H2O
-
Persephonella marina H2CO3
-
r
CO2 + H2O
-
Persephonella marina DSM 14350 / EX-H1 H2CO3
-
r
H2CO3
-
Persephonella marina CO2 + H2O
-
r
H2CO3
-
Persephonella marina DSM 14350 / EX-H1 CO2 + H2O
-
r
additional information enzyme activity measurement by determining the enzyme's CO2 hydration activity and esterase activity with substrate 4-nitrophenyl acetate. The recombinant enzyme lacking the signal peptide can efficiently catalyze the hydration of CO2, precipitating CaCO3 as calcite in the presence of Ca2+ Persephonella marina ?
-
?
additional information enzyme activity measurement by determining the enzyme's CO2 hydration activity and esterase activity with substrate 4-nitrophenyl acetate. The recombinant enzyme lacking the signal peptide can efficiently catalyze the hydration of CO2, precipitating CaCO3 as calcite in the presence of Ca2+ Persephonella marina DSM 14350 / EX-H1 ?
-
?

Subunits

Subunits Comment Organism
homodimer
-
Persephonella marina

Synonyms

Synonyms Comment Organism
alpha-type CA
-
Persephonella marina
PERMA_1443
-
Persephonella marina
PMCA
-
Persephonella marina

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
-
4 esterase assay at Persephonella marina
25
-
CO2hydration reaction, assay at Persephonella marina
35 45 CA-catalyzed sequestration of CO2 hydration, assay at Persephonella marina

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 100 purified recombinant His-tagged enzyme lacking the signal peptide, pH 7.6, 15 min, slow decrease in the enzyme activity at temperatures up to 50°C and almost no effect beyond 50°C up to 100°C. The enzyme retains over 80% of its activity between 60°C and 100°C, the Tm is 84.5°C Persephonella marina

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
320000
-
CO2 pH 7.8, 25°C, recombinant enzyme Persephonella marina

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
esterase assay at Persephonella marina
7.8 8.3 assay at, the pH drops during the CO2 hydration reaction to pH 6.2 Persephonella marina

pH Range

pH Minimum pH Maximum Comment Organism
4 10 activity range, recombinant enzyme Persephonella marina

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
30000
-
CO2 pH 7.8, 25°C, recombinant enzyme Persephonella marina