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Literature summary for 4.2.1.105 extracted from

  • Hakamatsuka, T.; Mori, K.; Ishida, S.; Ebizuka, Y.; Sankawa, U.
    Purification of 2-hydroxyisoflavanone dehydratase from the cell cultures of Pueraria lobata (1998), Phytochemistry, 49, 497-505.
No PubMed abstract available
Search for more literature for 4.2.1.105

Inhibitors

Inhibitors Comment Organism Structure
2,3-butadione 0.1 mM, complete inhibition Pueraria montana var. lobata
diethyldicarbonate 0.5 mM, 69% inhibition Pueraria montana var. lobata
iodoacetamide 50 mM, 56% inhibition Pueraria montana var. lobata
iodoacetic acid 50 mM, 43% inhibition Pueraria montana var. lobata
NEM 1.0 mM, 71% inhibition Pueraria montana var. lobata
PCMB 0.01 mM, 91% inhibition Pueraria montana var. lobata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7
-
 2,7,4'-trihydroxyisoflavanone
-
 Pueraria montana var. lobata

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Pueraria montana var. lobata
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 gel filtration Pueraria montana var. lobata
38000
-
 1 * 38000, SDS-PAGE Pueraria montana var. lobata

Organism

Organism UniProt Comment Textmining
Pueraria montana var. lobata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pueraria montana var. lobata

Reaction

Reaction Comment Organism Reaction ID
2,4',7-trihydroxyisoflavanone = daidzein + H2O (1) Pueraria montana var. lobata
a

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Pueraria montana var. lobata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.408
-
-
 Pueraria montana var. lobata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,7,4'-trihydroxyisoflavanone
-
 Pueraria montana var. lobata daidzein + H2O
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 38000, SDS-PAGE Pueraria montana var. lobata

Synonyms

Synonyms Comment Organism
2,7,4'-trihydroxyisoflavanone dehydratase
-
 Pueraria montana var. lobata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
 Pueraria montana var. lobata

pH Range

pH Minimum pH Maximum Comment Organism
6 7.9 half-maximal activity at pH 6.0 and pH 7.9 in potassium phosphate buffer Pueraria montana var. lobata

Cofactor

Cofactor Comment Organism Structure
additional information no cofactor required Pueraria montana var. lobata

pI Value

Organism Comment pI Value Maximum pI Value
Pueraria montana var. lobata isoelectric focusing, pH gradient 4-6.5
-
 4.9