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Literature summary for 4.2.1.112 extracted from
- Purification and characterization of acetylene hydratase of Pelobacter acetylenicus, a tungsten iron-sulfur protein (1995), J. Bacteriol., 177, 5767-5772.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Dithionite | a strong reductant is required for activity | Syntrophotalea acetylenica |  |
| additional information | no activation by tungstate at 5 mM | Syntrophotalea acetylenica | |
| Ti(III)citrate | a strong reductant is required for activity | Syntrophotalea acetylenica | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CO | 90% inhibition at 0.8 mM | Syntrophotalea acetylenica | |
| HgCl2 | reduces enzyme activity by 40% at 0.01 mM, 80% at 0.1 mM, and 98% at 0.2 mM | Syntrophotalea acetylenica | |
| KCN | 20% inhibition at 1 mM, 40% inhibition at 5-10 mM | Syntrophotalea acetylenica | |
| molybdate | - |
Syntrophotalea acetylenica | |
| additional information | no inhibition by citrate, ascorbate, tiron, ferrocene, and EDTA, no inhibition by ethylene at 8 mM | Syntrophotalea acetylenica | |
| nitric oxide | complete inhibition at 3 mM | Syntrophotalea acetylenica | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | the enzyme activity depends on the redox status, overview | Syntrophotalea acetylenica | |
| 0.014 | - |
Acetylene | pH 7.0, 30°C | Syntrophotalea acetylenica | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron-sulfur cluster | dependent on, the enzyme is a tungsten/iron-sulfur protein, 4.8 mol of iron per mol of enzyme and 3.9 mol od acid-labile sulfur per mol of enzyme | Syntrophotalea acetylenica | |
| additional information | no molybdenum detectable | Syntrophotalea acetylenica | |
| Tungsten | dependent on, the enzyme is a tungsten/iron-sulfur protein, 0.4 mol of tungsten per mol of enzyme | Syntrophotalea acetylenica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
gel filtration | Syntrophotalea acetylenica |
| 73000 | - |
1 * 73000, SDS-PAGE | Syntrophotalea acetylenica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetylene + H2O | Syntrophotalea acetylenica | - |
acetaldehyde | - |
? | |
| acetylene + H2O | Syntrophotalea acetylenica WoAcy1 / DSM 3246 | - |
acetaldehyde | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Syntrophotalea acetylenica | - |
- |
- |
| Syntrophotalea acetylenica WoAcy1 / DSM 3246 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| under air at room temperature, native enzyme 240-fold by ammonium sulfate fractionation, anion exchange chromatography, gel filtration, and a second anion exchange chromatography step to homogeneity | Syntrophotalea acetylenica |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition:acetylene-grown cell | - |
Syntrophotalea acetylenica | - |
| culture condition:molybdate-grown cell | - |
Syntrophotalea acetylenica | - |
| culture condition:tungstate-grown cell | - |
Syntrophotalea acetylenica | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 69.2 | - |
purified enzyme | Syntrophotalea acetylenica |
Storage Stability
| Storage Stability | Organism |
|---|---|
| enzyme activity is stable even after prolonged storage of the cell extract or of the purified protein under air | Syntrophotalea acetylenica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetylene + H2O | - |
Syntrophotalea acetylenica | acetaldehyde | - |
? | |
| acetylene + H2O | - |
Syntrophotalea acetylenica WoAcy1 / DSM 3246 | acetaldehyde | - |
? | |
| additional information | no activity with ethylene, methylene blue, or anthraquinone disulfonate, the purified enzyme has no coenzyme A-acetylating aldehyde dehydrogenase activity | Syntrophotalea acetylenica | ? | - |
? | |
| additional information | no activity with ethylene, methylene blue, or anthraquinone disulfonate, the purified enzyme has no coenzyme A-acetylating aldehyde dehydrogenase activity | Syntrophotalea acetylenica WoAcy1 / DSM 3246 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 73000, SDS-PAGE | Syntrophotalea acetylenica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Syntrophotalea acetylenica |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | 80 | - |
Syntrophotalea acetylenica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 6.5 | - |
Syntrophotalea acetylenica |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 9 | - |
Syntrophotalea acetylenica |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Syntrophotalea acetylenica | chromatofocusing | - |
4.3 |
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