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Literature summary for 4.2.1.113 extracted from

  • Glasner, M.E.; Fayazmanesh, N.; Chiang, R.A.; Sakai, A.; Jacobson, M.P.; Gerlt, J.A.; Babbitt, P.C.
    Evolution of structure and function in the o-succinylbenzoate synthase/N-acylamino acid racemase family of the enolase superfamily (2006), J. Mol. Biol., 360, 228-250.
    View publication on PubMed
Search for more literature for 4.2.1.113

Crystallization (Commentary)

Crystallization (Comment) Organism
striking differences in overall structure, altered oligomeric structure, length variation, orientation of the capping domain, active site structure, differences in substrate binding, conformation and position of the 20s loop structure, evolution of new protein functions by promiscuous binding and reactions with new substrates due to structural flexibility Amycolatopsis sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information substrate affinity is as much as 40fold higher for Escherichia coli OSBS than for OSBS of Amycolatopsis, also stated by analysis on phylogeny, structure, sequence conservation Amycolatopsis sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(1R,6R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate Amycolatopsis sp. menaquinone pathway 4-(2-carboxyphenyl)-4-oxobutanoic acid + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Amycolatopsis sp.
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 specificity maintained with little conservation of ligand-contacting residues despite of extensive structural variation in orthologous proteins Amycolatopsis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(1R,6R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate menaquinone pathway Amycolatopsis sp. 4-(2-carboxyphenyl)-4-oxobutanoic acid + H2O
-
 ?
(1R,6R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate menaquinone pathway, presence in species lacking the menaquinone pathway suggests additional roles in amino acid racemization Amycolatopsis sp. 4-(2-carboxyphenyl)-4-oxobutanoic acid + H2O
-
 ?

Synonyms

Synonyms Comment Organism
o-succinylbenzoate synthase
-
 Amycolatopsis sp.
OSBS
-
 Amycolatopsis sp.