Cloned (Comment) | Organism |
---|---|
AtECH2 contains a peroxisome targeting signal at the C-terminal end, is addressed to the peroxisome in Saccharomyces cerevisiae, and a fusion protein between AtECH2 and a fluorescent protein is targeted to peroxisomes in onion cells. To assess the peroxisomal addressing of AtECH2, a fusion protein between an EYFP at the N terminus and AtECH2 at the C terminus is constructed and expressed under the control of a double cauliflower mosaic virus (CaMV) 35 S viral promoter to allow transient expression of the fusion protein in onion cells following biolistic bombardment. The fluorescence is examined by confocal microscopy after 12 h | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisome | - |
Arabidopsis thaliana | 5777 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
x * 34000, calculated from sequence | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3R)-3-hydroxyacyl-CoA | Arabidopsis thaliana | AtECH2 participates in vivo in the conversion of the intermediate (3R)-hydroxyacyl-CoA, generated by the metabolism of fatty acids with a cis (Z)-unsaturated bond on an even-numbered carbon, to the (2E)-enoyl-CoA for further degradation through the core beta-oxidation cycle. AtECH2 is a monofunctional enzyme in Arabidopsis thaliana that is devoid of 3-hydroxyacyl-CoA dehydrogenase activity | (2E)-2-enoyl-CoA + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q8VYI3 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower bud | - |
Arabidopsis thaliana | - |
leaf | AtECH2 gene expression is strongest in tissues with high beta-oxidation activity, such as germinating seedlings and senescing leaves | Arabidopsis thaliana | - |
root | - |
Arabidopsis thaliana | - |
seedling | AtECH2 gene expression is strongest in tissues with high beta-oxidation activity, such as germinating seedlings and senescing leaves | Arabidopsis thaliana | - |
stem | - |
Arabidopsis thaliana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.2 | - |
0.03 mM (2E)-hexadecenoyl-CoA as a substrate | Arabidopsis thaliana |
3.5 | - |
0.03 m (2E)-hexenoyl-CoA as a substrate | Arabidopsis thaliana |
12.4 | - |
0.1 mM, 3-hydroxydecanoyl-CoA as a substrate | Arabidopsis thaliana |
30 | - |
0.05 mM (2E)-decenoyl-CoA as a substrate | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E)-decenoyl-CoA + H2O | - |
Arabidopsis thaliana | (3R)-3-hydroxydecanoyl-CoA | - |
r | |
(2E)-hexadecenoyl-CoA + H2O | - |
Arabidopsis thaliana | (3R)-3-hydroxyhexadecanoyl-CoA | - |
? | |
(2E)-hexenoyl-CoA + H2O | - |
Arabidopsis thaliana | (3R)-3-hydroxyhexanoyl-CoA | - |
? | |
(3R)-3-hydroxyacyl-CoA | AtECH2 participates in vivo in the conversion of the intermediate (3R)-hydroxyacyl-CoA, generated by the metabolism of fatty acids with a cis (Z)-unsaturated bond on an even-numbered carbon, to the (2E)-enoyl-CoA for further degradation through the core beta-oxidation cycle. AtECH2 is a monofunctional enzyme in Arabidopsis thaliana that is devoid of 3-hydroxyacyl-CoA dehydrogenase activity | Arabidopsis thaliana | (2E)-2-enoyl-CoA + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 34000, calculated from sequence | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
AtECH2 | gene name. Alignment of AtECH2 with homologous proteins is shown | Arabidopsis thaliana |
AtECH2 | monofunctional enzyme in Arabidopsis thaliana | Arabidopsis thaliana |
enoyl-CoA hydratase 2 | - |
Arabidopsis thaliana |
enoyl-CoA hydratase 2 | monofunctional enzyme in Arabidopsis thaliana | Arabidopsis thaliana |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Arabidopsis thaliana | calculated from sequence | - |
7.6 |