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Literature summary for 4.2.1.119 extracted from

  • Kashiwayama, Y.; Tomohiro, T.; Narita, K.; Suzumura, M.; Glumoff, T.; Hiltunen, J.K.; Van Veldhoven, P.P.; Hatanaka, Y.; Imanaka, T.
    Identification of a substrate-binding site in a peroxisomal beta-oxidation enzyme by photoaffinity labeling with a novel palmitoyl derivative (2010), J. Biol. Chem., 285, 26315-26325.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant C-terminally His6-tagged MFE2s in Escherichia coli Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
R251A site-directed mutagenesis, the mutant shows decreased labeling efficiency in photoaffinity labeling of substrate binding sites with photophores compared to the wild-type enzyme, overview Rattus norvegicus
W249A site-directed mutagenesis, the mutant shows decreased labeling efficiency in photoaffinity labeling of substrate binding sites with photophores compared to the wild-type enzyme, overview Rattus norvegicus
W249G site-directed mutagenesis, the mutant shows decreased labeling efficiency in photoaffinity labeling of substrate binding sites with photophores compared to the wild-type enzyme, overview Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
peroxisomal matrix MFE2 possesses a typical peroxisomal matrix protein targeting signal, COOH-terminal Ala-Lys-Leu Rattus norvegicus 5782
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Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
x * 80000, SDS-PAGE Rattus norvegicus
155000
-
recombinant enzyme, sucrose density gradient centrifugation Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
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Rattus norvegicus Wistar
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information identification of substrate binding sites, residues Trp249 to Arg251, using a photoreactive palmitic acid analogue bearing a diazirine moiety as a photophore in photoaffinity labeling of purified rat liver peroxisomes, ligand preparation, overview. The labeling efficiency competitively decreases in the presence of palmitoyl-CoA Rattus norvegicus ?
-
?
additional information identification of substrate binding sites, residues Trp249 to Arg251, using a photoreactive palmitic acid analogue bearing a diazirine moiety as a photophore in photoaffinity labeling of purified rat liver peroxisomes, ligand preparation, overview. The labeling efficiency competitively decreases in the presence of palmitoyl-CoA Rattus norvegicus Wistar ?
-
?

Subunits

Subunits Comment Organism
? x * 80000, SDS-PAGE Rattus norvegicus
More MFE2 consists of a (3R)-hydroxyacyl-CoA dehydrogenase, HD, domain, a (2E)-enoyl-CoA hydratase 2, H2, domain, and a sterol carrier protein 2-like domain Rattus norvegicus

Synonyms

Synonyms Comment Organism
MFE2
-
Rattus norvegicus
multifunctional enzyme type 2
-
Rattus norvegicus

General Information

General Information Comment Organism
metabolism the enzyme is involved in the peroxisomal beta-oxidation of fatty acids and their derivatives Rattus norvegicus
physiological function MFE2 consists of a (3R)-hydroxyacyl-CoA dehydrogenase, HD, domain, a (2E)-enoyl-CoA hydratase 2, H2, domain, and a sterol carrier protein 2-like domain, and is known to catalyze the second and third steps of the peroxisomal beta-oxidation of fatty acids and their derivatives Rattus norvegicus