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Literature summary for 4.2.1.119 extracted from

  • Tsuchida, S.; Kawamoto, K.; Endo, N.; Nunome, K.; Hamaue, N.; Aoki, T.
    Hydratase activities of green fluorescent protein tagged human multifunctional enzyme type 2 hydratase domain and its variants (2012), J. Oleo Sci., 61, 443-450.
    View publication on PubMed
Search for more literature for 4.2.1.119

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant hydratase domains of multifunctional enzyme type 2 hydratase as GFP-tagged protein in Escherichia coli strain JM109 Homo sapiens

Protein Variants

Protein Variants Comment Organism
A348T site-directed mutagenesis, the mutation does not affect the enzyme Homo sapiens
A427V site-directed mutagenesis, the mutation does not affect the enzyme, the mutant shows a slight increase in activity Homo sapiens
A491T site-directed mutagenesis, the mutation does not affect the enzyme Homo sapiens
A606S site-directed mutagenesis, the mutant shows reduced activity Homo sapiens
D510Y site-directed mutagenesis, inactive mutant, the mutation disrupts active site architecture Homo sapiens
E366G site-directed mutagenesis, inactive mutant, the mutation disrupts dimerization Homo sapiens
G533R site-directed mutagenesis, inactive mutant, the mutation disrupts ligand interaction Homo sapiens
H532R site-directed mutagenesis, inactive mutant, the mutation disrupts active site architecture Homo sapiens
I516T site-directed mutagenesis, the mutation disrupts dimerization, the mutant shows reduced activity Homo sapiens
I559V site-directed mutagenesis, the mutation does not affect the enzyme Homo sapiens
L405P site-directed mutagenesis, inactive mutant, the mutation disrupts ligand interaction Homo sapiens
additional information site-directed mutagenesis is used for construction of mutants corresponding to 17 reported missense mutations of multifunctional enzyme type 2 hydratase. Some mutants are almost or completely inactive causing the D-bifunctional protein deficiency type II Homo sapiens
N457D site-directed mutagenesis, the mutant shows reduced activity Homo sapiens
N457Y site-directed mutagenesis, the mutation disrupts domain folding, the mutant shows reduced activity Homo sapiens
P529L site-directed mutagenesis, inactive mutant, the mutation disrupts active site architecture Homo sapiens
R506C site-directed mutagenesis, inactive mutant, the mutation disrupts dimerization Homo sapiens
R506H site-directed mutagenesis, inactive mutant, the mutation disrupts dimerization Homo sapiens
W511R site-directed mutagenesis, the mutation does not affect the enzyme Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
peroxisome
-
 Homo sapiens 5777
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant GFP-tagged hydratase domains of multifunctional enzyme type 2 hydratase from Escherichia coli strain JM109 by anion exchange chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3R)-3-hydroxyhexadecanoyl-CoA
-
 Homo sapiens (2E)-2-hexadecenoyl-CoA + H2O
-
 ?

Synonyms

Synonyms Comment Organism
MFE2 hydratase
-
 Homo sapiens
multifunctional enzyme type 2 hydratase
-
 Homo sapiens

General Information

General Information Comment Organism
malfunction inactivating mutations of multifunctional enzyme type 2 hydratase lead to D-bifunctional protein deficiency type II Homo sapiens
additional information MFE2 hydratase is R-specific, while MFE1 hydratase is S-specific Homo sapiens