Cloned (Comment) | Organism |
---|---|
gene phaJ_1, DNA and amino acid sequence determination and analysis, sequence comparisons, the gene encoding MaoC-like protein locates in the pha cluster, functional recombinant expression in Escherichia coli strains DH5alpha and LS5218 | Bacillus cereus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | in an in vivo assay using Escherichia coli as a host for polyhydroxyalkanoate (PHA) accumulation, the recombinant strain expressing PhaJYB4 and PHA synthase leads to increased PHA accumulation, suggesting that PhaJYB4 functioned as a monomer supplier. The pha cluster from Bacillus cereus strain YB-4 functions to accumulate PHA in Escherichia coli, but it does not function when the phaJYB4 gene is deleted. The recombinant strain utilizes butyrate, valerate, hexanoate, and dodecanoate for PHA synthesis | Bacillus cereus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E)-2-enoyl-CoA + H2O | Bacillus cereus | - |
(3R)-3-hydroxyacyl-CoA | - |
r | |
(2E)-2-enoyl-CoA + H2O | Bacillus cereus YB-4 | - |
(3R)-3-hydroxyacyl-CoA | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | A0A150CP14 | - |
- |
Bacillus cereus YB-4 | A0A150CP14 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E)-2-enoyl-CoA + H2O | - |
Bacillus cereus | (3R)-3-hydroxyacyl-CoA | - |
r | |
(2E)-2-enoyl-CoA + H2O | - |
Bacillus cereus YB-4 | (3R)-3-hydroxyacyl-CoA | - |
r | |
(2E)-crotonyl-CoA + H2O | - |
Bacillus cereus | (3R)-3-hydroxybutanoyl-CoA | - |
r | |
(2E)-crotonyl-CoA + H2O | - |
Bacillus cereus YB-4 | (3R)-3-hydroxybutanoyl-CoA | - |
r | |
additional information | PhaJYB4 activity is thought to be specific for short chain-length enoyl-CoA | Bacillus cereus | ? | - |
? | |
additional information | PhaJYB4 activity is thought to be specific for short chain-length enoyl-CoA | Bacillus cereus YB-4 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MaoC-like protein | - |
Bacillus cereus |
phaJ | - |
Bacillus cereus |
PhaJYB4 | - |
Bacillus cereus |
R-hydratase | - |
Bacillus cereus |
R-specific enoyl-CoA hydratase | - |
Bacillus cereus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Bacillus cereus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Bacillus cereus |
General Information | Comment | Organism |
---|---|---|
evolution | enzyme PhaJYB4 shows a high homology to short chain-length (C4-C6)-specific PhaJs, e.g. Pseudomonas aeruginosa PhaJ1Pa and Aeromonas caviae PhaJAc, rather than medium chain-length (C8-C12)-specific PhaJs, e.g. Pseudomonas aeruginosa PhaJ4Pa4 | Bacillus cereus |
physiological function | Bacillus cereus accumulates polyhydroxyalkanoate (PHA). The MaoC-like protein has an R-specific enoyl-CoA hydratase activity and is referred to as PhaJ when involved in the PHA metabolism. In an in vivo assay using Escherichia coli as a host for PHA accumulation, the recombinant strain expressing PhaJYB4 and PHA synthase leads to increased PHA accumulation, suggesting that PhaJYB4 functioned as a monomer supplier | Bacillus cereus |