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Literature summary for 4.2.1.17 extracted from

  • Kiema, T.R.; Engel, C.K.; Schmitz, W.; Filppula, S.A.; Wierenga, R.K.; Hiltunen, J.K.
    Mutagenic and enzymological studies of the hydratase and isomerase activities of 2-enoyl-CoA hydratase-1 (1999), Biochemistry, 38, 2991-2999.
    View publication on PubMed
Search for more literature for 4.2.1.17

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
E144A kcat for trans-2-hexenoyl-CoA is 1733fold lower than wild-type value Rattus norvegicus
E144A/Q162L kcat for trans-2-hexenoyl-CoA is 12417fold lower than wild-type value. The point mutations E144A and Q162L by themselves apparently do not cause structural rearrangements of the active site helix, but when both residues are changed, the active site geometry changes Rattus norvegicus
E164A kcat for trans-2-hexenoyl-CoA is 1709fold lower than wild-type value Rattus norvegicus
Q162A kcat for trans-2-hexenoyl-CoA is nearly identical to wild-type value Rattus norvegicus
Q162L kcat for trans-2-hexenoyl-CoA is nearly identical to wild-type value Rattus norvegicus
Q162M kcat for trans-2-hexenoyl-CoA is nearly identical to wild-type value Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0025
-
 trans-2-decenoyl-CoA pH 8.0, 22°C, mutant enzyme E144A/Q162L Rattus norvegicus
0.0029
-
 trans-2-decenoyl-CoA pH 8.0, 22°C, mutant enzyme Q162A Rattus norvegicus
0.003
-
 trans-2-decenoyl-CoA pH 8.0, 22°C, mutant enzyme E144A Rattus norvegicus
0.0039
-
 trans-2-decenoyl-CoA pH 8.0, 22°C, wild-type enzyme Rattus norvegicus
0.005
-
 trans-2-decenoyl-CoA pH 8.0, 22°C, mutant enzyme Q162L Rattus norvegicus
0.0052
-
 trans-2-decenoyl-CoA pH 8.0, 22°C, mutant enzyme E164A Rattus norvegicus
0.0063
-
 trans-2-decenoyl-CoA pH 8.0, 22°C, mutant enzyme Q162M Rattus norvegicus
0.0143
-
 trans-2-hexenoyl-CoA pH 8.0, 22°C, mutant enzyme Q162A Rattus norvegicus
0.0152
-
 trans-2-hexenoyl-CoA pH 8.0, 22°C, mutant enzyme E144A Rattus norvegicus
0.021
-
 trans-2-hexenoyl-CoA pH 8.0, 22°C, mutant enzyme E144A/Q162L Rattus norvegicus
0.0229
-
 trans-2-hexenoyl-CoA pH 8.0, 22°C, mutant enzyme Q162M Rattus norvegicus
0.024
-
 trans-2-hexenoyl-CoA pH 8.0, 22°C, mutant enzyme E164A Rattus norvegicus
0.025
-
 trans-2-hexenoyl-CoA pH 8.0, 22°C, wild-type enzyme Rattus norvegicus
0.027
-
 trans-2-hexenoyl-CoA pH 8.0, 22°C, mutant enzyme Q162L Rattus norvegicus
0.0499
-
 crotonyl-CoA pH 8.0, 22°C, wild-type enzyme Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Rattus norvegicus 5739
-

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
64
-
 homogenate of cells transformed with the pET3aECH1 expression vector Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
crotonyl-CoA + H2O
-
 Rattus norvegicus (3S)-3-hydroxybutanoyl-CoA
-
 ?
additional information the enzyme also catalyzes DELTA3-DELTA2-isomerization of trans-3-hexenoyl-CoA Rattus norvegicus ?
-
 ?
trans-2-decenoyl-CoA + H2O
-
 Rattus norvegicus (3S)-hydroxydecanoyl-CoA
-
 ?
trans-2-hexenoyl-CoA + H2O
-
 Rattus norvegicus (3S)-3-hydroxyhexanoyl-CoA
-
 ?

Synonyms

Synonyms Comment Organism
2-enoyl-CoA hydratase-1
-
 Rattus norvegicus
crotonase
-
 Rattus norvegicus
mECH-1
-
 Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0121
-
 trans-2-decenoyl-CoA pH 8.0, 22°C, mutant enzyme E144A/Q162L Rattus norvegicus
0.06
-
 trans-2-hexenoyl-CoA pH 8.0, 22°C, mutant enzyme E144A/Q162L Rattus norvegicus
0.085
-
 trans-2-decenoyl-CoA pH 8.0, 22°C, mutant enzyme E144A Rattus norvegicus
0.095
-
 trans-2-decenoyl-CoA pH 8.0, 22°C, mutant enzyme E164A Rattus norvegicus
0.43
-
 trans-2-hexenoyl-CoA pH 8.0, 22°C, mutant enzyme E144A Rattus norvegicus
0.44
-
 trans-2-hexenoyl-CoA pH 8.0, 22°C, mutant enzyme E164A Rattus norvegicus
104
-
 trans-2-decenoyl-CoA pH 8.0, 22°C, mutant enzyme Q162A Rattus norvegicus
164
-
 trans-2-decenoyl-CoA pH 8.0, 22°C, mutant enzyme Q162M Rattus norvegicus
174
-
 trans-2-decenoyl-CoA pH 8.0, 22°C, mutant enzyme Q162L Rattus norvegicus
203
-
 trans-2-decenoyl-CoA pH 8.0, 22°C, wild-type enzyme Rattus norvegicus
561
-
 trans-2-hexenoyl-CoA pH 8.0, 22°C, mutant enzyme Q162L Rattus norvegicus
601
-
 trans-2-hexenoyl-CoA pH 8.0, 22°C, mutant enzyme Q162M Rattus norvegicus
607
-
 trans-2-hexenoyl-CoA pH 8.0, 22°C, mutant enzyme Q162A Rattus norvegicus
745
-
 trans-2-hexenoyl-CoA pH 8.0, 22°C, wild-type enzyme Rattus norvegicus
2238
-
 crotonyl-CoA pH 8.0, 22°C, wild-type enzyme Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Rattus norvegicus