Activating Compound | Comment | Organism | Structure |
---|---|---|---|
CoA | the enzyme is dependent on CoA | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
E144A | site-directed mutagenesis, the mutant shows a 1000fold reduced activity compared to the wild-type enzyme | Rattus norvegicus |
E164A | site-directed mutagenesis, the mutant shows a 1000fold reduced activity compared to the wild-type enzyme | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | ECH catalyzes the reversible syn-addition of a water molecule across the double bond of a trans-2-enoyl-CoA, e.g. crotonyl-CoA, thioester to give a beta-hydroxyacyl-CoA thioester. The enzyme binds the substrates at the interface between monomers within the same trimer | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P14604 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
crotonyl-CoA + H2O | stereoselective reaction mechanism, Glu144 and Glu164 are essential for ECH catalysis, overview | Rattus norvegicus | (3S)-3-hydroxybutanoyl-CoA | - |
? | |
additional information | ECH catalyzes the reversible syn-addition of a water molecule across the double bond of a trans-2-enoyl-CoA, e.g. crotonyl-CoA, thioester to give a beta-hydroxyacyl-CoA thioester. The enzyme binds the substrates at the interface between monomers within the same trimer | Rattus norvegicus | ? | - |
? | |
additional information | ECH (XI) also has enoyl-CoA isomerase activity at approximately 1/5000 the level of its hydratase activity, overview | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme binds the substrates at the interface between monomers within the same trimer, monomer structure, modelling in comparison to other crotonase superfamily enzymes, overview | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
ECH | - |
Rattus norvegicus |
More | crotonase superfamily enzyme | Rattus norvegicus |