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Literature summary for 4.2.1.17 extracted from
- Structural and functional characterization of the recombinant human mitochondrial trifunctional protein (2010), Biochemistry, 49, 8608-8617.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| co-expression of His-tagged alpha- and beta-subunits in Escherichia coli strain Rosetta-2(DE3) | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme is controlled by feed-back inhibition, overview | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrial inner membrane | - |
Homo sapiens | 5743 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 49291 | - |
x * 79014, alpha-subunit, + x * 49291, beta-subunit, mass spectrometry and gel filtration | Homo sapiens |
| 79014 | - |
x * 79014, alpha-subunit, + x * 49291, beta-subunit, mass spectrometry and gel filtration | Homo sapiens |
| 460000 | - |
about, enoyl-CoA hydratase complex, gel filtration | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant alpha- and His-tagged beta-subunits from Escherichia coli by nickel affinity chromatography to homogeneity | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | recombinant TFP interacts strongly with cardiolipin and phosphatidylcholine | Homo sapiens | ? | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | the alpha and beta subunits of the human mitochondrial trifunctional protein enoyl-CoA hydratase are part of the multienzyme complex, with predominance of alpha2beta2 and alpha4beta4 complexes, with higher order oligomers | Homo sapiens |
| oligomer | x * 79014, alpha-subunit, + x * 49291, beta-subunit, mass spectrometry and gel filtration | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 2-enoyl-CoA hydratase | - |
Homo sapiens |
| ECH | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
assay at | Homo sapiens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the human mitochondrial trifunctional protein enoyl-CoA hydratase is a multienzyme complex involved in fatty acid beta-oxidation. The pathway shows feed-back inhibition, overview | Homo sapiens |
| physiological function | recombinant enoyl-CoA hydratase displays 2-enoyl-CoA hydratase, L-3-hydroxyacyl-CoA dehydrogenase, EC 1.1.1.35, and 3-ketoacyl-CoA thiolase, EC 2.3.1.16, activities | Homo sapiens |
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