Literature summary for 4.2.1.17 extracted from

Amari, D.T.; Marques, C.N.; Davies, D.G.
The putative enoyl-coenzyme A hydratase DspI is required for production of the Pseudomonas aeruginosa biofilm dispersion autoinducer cis-2-decenoic acid (2013), J. Bacteriol., 195, 4600-4610.

Search for more literature for 4.2.1.17

Cloned(Commentary)

Cloned (Comment)	Organism
gene dspI, quantitative reverse transcriptase PCR expression analysis. Gene dspI is co-transcribed with the upstream genes PA14_54620 and PA14_54630	Pseudomonas aeruginosa

Protein Variants

Protein Variants	Comment	Organism
additional information	generation of a deletion mutant strain DELTAdspI, functional complementation of DELTAdspI, overview	Pseudomonas aeruginosa

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	Q9I5I4	gene dspI or PA14_54640 (PA0745)	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	high expression levels of dspI in planktonic and biofilm cells	Pseudomonas aeruginosa	-

Synonyms

Synonyms	Comment	Organism
enoyl-CoA hydratase/isomerase	-	Pseudomonas aeruginosa
enoyl-coenzyme A hydratase/isomerase	-	Pseudomonas aeruginosa

General Information

General Information	Comment	Organism
malfunction	inactivation of dspI abolishes biofilm dispersion autoinduction in continuous cultures of Pseudommonas aeruginosa and results in biofilms that are significantly greater in thickness and biomass compared to the parental wild-type strain. But dispersion can be induced in dspI mutants by the exogenous addition of synthetic cis-2-decenoic acid or by complementation of DELTAdspI in trans under the control of an arabinose-inducible promoter	Pseudomonas aeruginosa
additional information	transcript abundance of dspI correlates with cell density	Pseudomonas aeruginosa
physiological function	the enzyme is responsible for catalyzing the formation of alpha,beta-unsaturated fatty acids and dspI is essential for production of cis-2-decenoic acid, and it is required for synthesis of the biofilm dispersion autoinducer cis-2-decenoic acid in the human pathogen Pseudomonas aeruginosa. Expression of dspI is correlated with cell density during planktonic and biofilm growth	Pseudomonas aeruginosa

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Release 2023.1 (January 2023)