Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-Amino-1,2,4-triazole | Ki: 0.03 mM | Saccharomyces cerevisiae | |
phosphate | Ki: 0.008 mM | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate | wild-type enzyme | Saccharomyces cerevisiae | |
0.3 | - |
D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate | expressed in E. coli | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | activity dependent on | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate | - |
Saccharomyces cerevisiae | 3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
wild-type enzyme | Saccharomyces cerevisiae |
8 | - |
enzyme expressed in Escherichia coli | Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.8 | 8 | wild-type enzyme, less than 50% of maximal activity above and below | Saccharomyces cerevisiae |
7.2 | 8.2 | enzyme expressed in E. coli, less than 50% of maximal activity above and below | Saccharomyces cerevisiae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
phosphate | - |
Saccharomyces cerevisiae | |
0.03 | - |
3-Amino-1,2,4-triazole | - |
Saccharomyces cerevisiae |