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Literature summary for 4.2.1.19 extracted from

  • Ahangar, M.S.; Vyas, R.; Nasir, N.; Biswal, B.K.
    Structures of native, substrate-bound and inhibited forms of Mycobacterium tuberculosis imidazoleglycerol-phosphate dehydratase (2013), Acta Crystallogr. Sect. D, 69, 2461-2467.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Mycobacterium smegmatis strain mc24517 Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
3-Amino-1,2,4-triazole competitive Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.122
-
D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate at pH 7.7 and 30°C Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ contains two Mn2+ ions, 0.05 mM Mn2+ used in assay conditions Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
570000
-
gel filtration Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate Mycobacterium tuberculosis
-
3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O
-
?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA affinity column chromatography and gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate
-
Mycobacterium tuberculosis 3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O
-
?

Subunits

Subunits Comment Organism
tetradodecamer
-
Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
IGPD
-
Mycobacterium tuberculosis
HisB
-
Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1000
-
D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate at pH 7.7 and 30°C Mycobacterium tuberculosis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.31
-
3-Amino-1,2,4-triazole at pH 7.7 and 30°C Mycobacterium tuberculosis