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Literature summary for 4.2.1.19 extracted from
- Structures of native, substrate-bound and inhibited forms of Mycobacterium tuberculosis imidazoleglycerol-phosphate dehydratase (2013), Acta Crystallogr. Sect. D, 69, 2461-2467.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Mycobacterium smegmatis strain mc24517 | Mycobacterium tuberculosis |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Mycobacterium tuberculosis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-Amino-1,2,4-triazole | competitive | Mycobacterium tuberculosis |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.122 | - |
D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate | at pH 7.7 and 30°C | Mycobacterium tuberculosis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | contains two Mn2+ ions, 0.05 mM Mn2+ used in assay conditions | Mycobacterium tuberculosis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 570000 | - |
gel filtration | Mycobacterium tuberculosis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate | Mycobacterium tuberculosis | - |
3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA affinity column chromatography and gel filtration | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate | - |
Mycobacterium tuberculosis | 3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetradodecamer | - |
Mycobacterium tuberculosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HisB | - |
Mycobacterium tuberculosis |
| IGPD | - |
Mycobacterium tuberculosis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1000 | - |
D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate | at pH 7.7 and 30°C | Mycobacterium tuberculosis | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.31 | - |
3-Amino-1,2,4-triazole | at pH 7.7 and 30°C | Mycobacterium tuberculosis | |
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Release 2023.1 (January 2023)
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