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Literature summary for 4.2.1.20 extracted from
- Mutational scanning of a hairpin loop in the tryptophan synthase beta-subunit implicated in allostery and substrate channeling (2000), Biol. Chem., 381, 1185-1193.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| F280A | beta-subunit dimers, unaltered activity compared to the wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
| F280A | beta-subunit dimers, unaltered activity compared to the wild-type enzyme | Escherichia coli |
| F280G | beta-subunit dimers, 72% reduced activity compared to the wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
| F280G | beta-subunit dimers, 72% reduced activity compared to the wild-type enzyme | Escherichia coli |
| F280P | beta-subunit dimers, 94% reduced activity compared to the wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
| F280P | beta-subunit dimers, 94% reduced activity compared to the wild-type enzyme | Escherichia coli |
| G281A | beta-subunit dimers, 46% reduced activity compared to the wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
| G281A | beta-subunit dimers, 46% reduced activity compared to the wild-type enzyme | Escherichia coli |
| H273A | beta-subunit dimers, 2fold increased activity compared to the wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
| H273A | beta-subunit dimers, 2fold increased activity compared to the wild-type enzyme | Escherichia coli |
| I278A | beta-subunit dimers, 70% reduced activity compared to the wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
| I278A | beta-subunit dimers, 70% reduced activity compared to the wild-type enzyme | Escherichia coli |
| I278A/K283A | beta-subunit dimers, 92% reduced activity compared to the wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
| I278A/K283A | beta-subunit dimers, 92% reduced activity compared to the wild-type enzyme | Escherichia coli |
| I278V | beta-subunit dimers, 7% reduced activity compared to the wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
| I278V | beta-subunit dimers, 7% reduced activity compared to the wild-type enzyme | Escherichia coli |
| I278V/K283A | beta-subunit dimers, 35% reduced activity compared to the wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
| I278V/K283A | beta-subunit dimers, 35% reduced activity compared to the wild-type enzyme | Escherichia coli |
| K283A | beta-subunit dimers, 41% reduced activity compared to the wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
| K283A | beta-subunit dimers, 41% reduced activity compared to the wild-type enzyme | Escherichia coli |
| M282A | beta-subunit dimers, 61% reduced activity compared to the wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
| M282A | beta-subunit dimers, 61% reduced activity compared to the wild-type enzyme | Escherichia coli |
| M282P | beta-subunit dimers, inactive mutant | Salmonella enterica subsp. enterica serovar Typhimurium |
| M282P | beta-subunit dimers, inactive mutant | Escherichia coli |
| R275A | beta-subunit dimers, 37% reduced activity compared to the wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
| R275A | beta-subunit dimers, 37% reduced activity compared to the wild-type enzyme | Escherichia coli |
| V276A | beta-subunit dimers, 46% reduced activity compared to the wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
| V276A | beta-subunit dimers, 46% reduced activity compared to the wild-type enzyme | Escherichia coli |
| V276A/K283A | beta-subunit dimers, 48% reduced activity compared to the wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
| V276A/K283A | beta-subunit dimers, 48% reduced activity compared to the wild-type enzyme | Escherichia coli |
| Y279A | beta-subunit dimers, 50% reduced activity compared to the wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
| Y279A | beta-subunit dimers, 50% reduced activity compared to the wild-type enzyme | Escherichia coli |
| Y279F | beta-subunit dimers, 33% reduced activity compared to the wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
| Y279F | beta-subunit dimers, 33% reduced activity compared to the wild-type enzyme | Escherichia coli |
| Y279L | beta-subunit dimers, 48% reduced activity compared to the wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
| Y279L | beta-subunit dimers, 48% reduced activity compared to the wild-type enzyme | Escherichia coli |
| Y279P | beta-subunit dimers, 46% reduced activity compared to the wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
| Y279P | beta-subunit dimers, 46% reduced activity compared to the wild-type enzyme | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O | also catalyses the conversion of serine and indole into tryptophan and water, and of indoleglycerol phosphate into indole and glyceraldehyde phosphate (the latter reaction was listed formerly as EC 4.2.1.8), allostery and substrate channeling | Salmonella enterica subsp. enterica serovar Typhimurium | |
| L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O | also catalyses the conversion of serine and indole into tryptophan and water, and of indoleglycerol phosphate into indole and glyceraldehyde phosphate (the latter reaction was listed formerly as EC 4.2.1.8), allostery and substrate channeling | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1524 | - |
wild-type enzyme in presence of alpha-subunit | Salmonella enterica subsp. enterica serovar Typhimurium |
| 1524 | - |
wild-type enzyme in presence of alpha-subunit | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-(indol-3-yl)glycerol 3-phosphate | alpha-subunit of the bienzyme complex, alpha-reaction | Salmonella enterica subsp. enterica serovar Typhimurium | D-glyceraldehyde 3-phosphate + indole | - |
? |  |
| 1-(indol-3-yl)glycerol 3-phosphate | alpha-subunit of the bienzyme complex, alpha-reaction | Escherichia coli | D-glyceraldehyde 3-phosphate + indole | - |
? | |
| L-serine + indole | beta-subunit of the bienzyme complex, beta-reaction | Salmonella enterica subsp. enterica serovar Typhimurium | L-tryptophan + H2O | - |
? | |
| L-serine + indole | beta-subunit of the bienzyme complex, beta-reaction | Escherichia coli | L-tryptophan + H2O | - |
? | |
| additional information | allostery and substrate channeling | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
| additional information | allostery and substrate channeling | Escherichia coli | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | structural analysis, wild-type and mutant beta-subunit dimers, hairpin loop in the beta-subunit, overview | Salmonella enterica subsp. enterica serovar Typhimurium |
| More | structural analysis, wild-type and mutant beta-subunit dimers, hairpin loop in the beta-subunit, overview | Escherichia coli |
| tetramer | alpha2beta2 complex | Salmonella enterica subsp. enterica serovar Typhimurium |
| tetramer | alpha2beta2 complex | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TSase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
| TSase | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
| 25 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
| 7.8 | - |
assay at | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| pyridoxal 5'-phosphate | - |
Escherichia coli | |
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Release 2023.1 (January 2023)
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