Application | Comment | Organism |
---|---|---|
analysis | the enzyme complex is a model enzyme for understanding allosteric regulation | Salmonella enterica subsp. enterica serovar Typhimurium |
Cloned (Comment) | Organism |
---|---|
expressionof mutant S178P in Escherichia coli | Salmonella enterica subsp. enterica serovar Typhimurium |
Crystallization (Comment) | Organism |
---|---|
hanging drop method, 10 mg/ml purified recombinant mutant S178P enzyme in 50 mM bicine, pH 7.8, with equal volume of reservoir solution: 50 mM bicine, 1 mM EDTA, 12% w/v PEG 8000, 1.4 mM spermine, pH 7.8, 21°C, structure determination and functional investigation by polarized absorption microspectrophotometry | Salmonella enterica subsp. enterica serovar Typhimurium |
Protein Variants | Comment | Organism |
---|---|---|
S178P | site-directed mutagenesis, molecular modeling of the structural effects caused by the mutation, hydrogen bond with alphaGlu181 is interrupted, 2fold decreased activity of the beta-subunit, not affected, in contrast to the wild-type, by allosteric effectors indole-3-acetylglycine and DL-alpha-glycerol-3-phosphate in the beta-reaction, weaker binding of Na+ | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant mutant S178P from Escherichia coli | Salmonella enterica subsp. enterica serovar Typhimurium |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O | also catalyses the conversion of serine and indole into tryptophan and water, and of indoleglycerol phosphate into indole and glyceraldehyde phosphate (the latter reaction was listed formerly as EC 4.2.1.8) | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(indol-3-yl)glycerol 3-phosphate | alpha-subunit of the bienzyme complex, alpha-reaction | Salmonella enterica subsp. enterica serovar Typhimurium | D-glyceraldehyde 3-phosphate + indole | - |
? | |
L-serine + indole | beta-subunit of the bienzyme complex, beta-reaction | Salmonella enterica subsp. enterica serovar Typhimurium | L-tryptophan + H2O | - |
? | |
additional information | allosteric communication, Ser178 is a critical residue in ligand-triggered signals between alpha and beta active sites | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | alpha2beta2 complex | Salmonella enterica subsp. enterica serovar Typhimurium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium |