Cloned (Comment) | Organism |
---|---|
genes trpA encoding the alpha-subunit and trpB encoding the beta-subunit are coexpressed or expressed individually in Escherichia coli strain JM109 | Pyrococcus furiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | thermodynamic parameters of subunit association, kinetics | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27500 | - |
alpha-subunit monomer, ultracentrifugation sedimentation equilibrium | Pyrococcus furiosus |
42500 | - |
2 * 275000 + 2 * 42500, recombinant alpha2beta2 enzyme complex, SDS-PAGE | Pyrococcus furiosus |
42500 | - |
2 * 42500, recombinant beta-subunit, SDS-PAGE | Pyrococcus furiosus |
84000 | 88000 | beta-subunit dimer, ultracentrifugation sedimentation equilibrium | Pyrococcus furiosus |
140000 | - |
enzyme complex alpha2beta2, ultracentrifugation sedimentation equilibrium | Pyrococcus furiosus |
275000 | - |
2 * 275000 + 2 * 42500, recombinant alpha2beta2 enzyme complex, SDS-PAGE | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant alpha-subunit, beta-subunit, and enzyme complex expressed in Escherichia coli | Pyrococcus furiosus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O | also catalyses the conversion of serine and indole into tryptophan and water, and of indoleglycerol phosphate into indole and glyceraldehyde phosphate (the latter reaction was listed formerly as EC 4.2.1.8) | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(indol-3-yl)glycerol 3-phosphate | alpha-subunit of the bienzyme complex, alpha-reaction | Pyrococcus furiosus | D-glyceraldehyde 3-phosphate + indole | - |
? | |
L-serine + indole | beta-subunit of the bienzyme complex, beta-reaction | Pyrococcus furiosus | L-tryptophan + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 42500, recombinant beta-subunit, SDS-PAGE | Pyrococcus furiosus |
monomer | 1 * 27500, recombinant alpha-subunit, SDS-PAGE | Pyrococcus furiosus |
More | high association constant for the hyperthermophile enzyme subunits, pH-dependence of the molecular weights | Pyrococcus furiosus |
tetramer | 2 * 275000 + 2 * 42500, recombinant alpha2beta2 enzyme complex, SDS-PAGE | Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
inactivation temperature of the alpha-subunit is enhanced by the formation of the enzyme complex alpha2beta2 by 17°C | Pyrococcus furiosus |