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Literature summary for 4.2.1.28 extracted from

  • Toraya, T.; Fukui, S.
    Coenzyme B12-dependent propanediol dehydratase system. Ternary complex between apoenzyme, coenzyme, and substrate analog (1972), Biochim. Biophys. Acta, 284, 536-548.
    View publication on PubMed
Search for more literature for 4.2.1.28

General Stability

General Stability Organism
ternary complex photostable, coenzyme alone photolabile Klebsiella pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
2,3-Butanediol competitive inhibitor, Ki: 2.08 mM Klebsiella pneumoniae
iodoacetamide apoenzyme completely inactivated Klebsiella pneumoniae
N-ethylmaleimide apoenzyme completely inactivated Klebsiella pneumoniae
p-chloromercuribenzoate apoenzyme completely inactivated Klebsiella pneumoniae
p-hydroxymercuribenzoate ternary complex stable, apoenzyme unstable Klebsiella pneumoniae
styrene glycol competitive inhibitor, Ki: 50.2 mM Klebsiella pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0814
-
 1,2-propanediol
-
 Klebsiella pneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
K+ stabilizes apoenzyme-coenzyme association Klebsiella pneumoniae

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
 formerly Aerobacter aerogenes
-

Oxidation Stability

Oxidation Stability Organism
apoenzyme-coenzyme-substrate-analog complex relatively stable to O2 compared with holoenzyme, analogues: 1,2-butanediol, styrene glycol, and others to a lesser extent Klebsiella pneumoniae

Reaction

Reaction Comment Organism Reaction ID
propane-1,2-diol = propanal + H2O possible role of sulfhydryl groups Klebsiella pneumoniae
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-butanediol formation of ternary complex between apoenzyme, coenzyme and substrate analogue Klebsiella pneumoniae butyraldehyde
-
 ?
1,2-ethanediol
-
 Klebsiella pneumoniae acetaldehyde
-
 ?
1,2-propanediol
-
 Klebsiella pneumoniae propanal
-
 ?
additional information substrate stabilizes apoenzyme-coenzyme association Klebsiella pneumoniae ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 apoenzyme labile above Klebsiella pneumoniae
50
-
 40% loss of activity of ternary complex after 5 min Klebsiella pneumoniae

Cofactor

Cofactor Comment Organism Structure
cobamide
-
 Klebsiella pneumoniae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
50.2
-
 styrene glycol competitive inhibitor Klebsiella pneumoniae