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Literature summary for 4.2.1.28 extracted from

  • Shibata, N.; Sueyoshi, Y.; Higuchi, Y.; Toraya, T.
    Direct participation of a peripheral side chain of a corrin ring in coenzyme B12 catalysis (2018), Angew. Chem. Int. Ed. Engl., 57, 7830-7835 .
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
structures in the absence and presence of substrates at resolutions of 1.55-2.05 A Klebsiella oxytoca

Protein Variants

Protein Variants Comment Organism
S224A mutation in alpha-subunit, a key residue for stabilizing the post-homolysis state of the adenosyl group. More than 5fold reduction in kcat/Km Klebsiella oxytoca
S224C mutation in alpha-subunit, a key residue for stabilizing the post-homolysis state of the adenosyl group. About 100fold reduction in kcat/Km Klebsiella oxytoca
S224N mutation in alpha-subunit, a key residue for stabilizing the post-homolysis state of the adenosyl group. More than 100fold reduction in kcat/Km Klebsiella oxytoca
T172A mutation in alpha-subunit, a key residue for stabilizing the post-homolysis state of the adenosyl group. More than 100fold reduction in kcat/Km Klebsiella oxytoca
T172S mutation in alpha-subunit, a key residue for stabilizing the post-homolysis state of the adenosyl group. More than 10fold reduction in kcat/Km Klebsiella oxytoca

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.031
-
Propane-1,2-diol mutant T172A, pH not specified in the publication, temperature not specified in the publication Klebsiella oxytoca
0.15
-
Propane-1,2-diol wild-type, pH not specified in the publication, temperature not specified in the publication Klebsiella oxytoca
0.15
-
Propane-1,2-diol mutant S224A, pH not specified in the publication, temperature not specified in the publication Klebsiella oxytoca
0.16
-
Propane-1,2-diol mutant T172S, pH not specified in the publication, temperature not specified in the publication Klebsiella oxytoca
0.51
-
Propane-1,2-diol mutant S224C, pH not specified in the publication, temperature not specified in the publication Klebsiella oxytoca
1.9
-
Propane-1,2-diol mutant S224N, pH not specified in the publication, temperature not specified in the publication Klebsiella oxytoca

Organism

Organism UniProt Comment Textmining
Klebsiella oxytoca Q59470 alpha subunit
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
propane-1,2-diol
-
Klebsiella oxytoca propanal + H2O
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.5
-
Propane-1,2-diol mutant T172A, pH not specified in the publication, temperature not specified in the publication Klebsiella oxytoca
12
-
Propane-1,2-diol mutant S224C, pH not specified in the publication, temperature not specified in the publication Klebsiella oxytoca
17
-
Propane-1,2-diol mutant S224N, pH not specified in the publication, temperature not specified in the publication Klebsiella oxytoca
34
-
Propane-1,2-diol mutant T172S, pH not specified in the publication, temperature not specified in the publication Klebsiella oxytoca
64
-
Propane-1,2-diol mutant S224A, pH not specified in the publication, temperature not specified in the publication Klebsiella oxytoca
336
-
Propane-1,2-diol wild-type, pH not specified in the publication, temperature not specified in the publication Klebsiella oxytoca

Cofactor

Cofactor Comment Organism Structure
adenosylcobalamin Km value 0.15 mM for wild-type Klebsiella oxytoca

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
9
-
Propane-1,2-diol mutant S224N, pH not specified in the publication, temperature not specified in the publication Klebsiella oxytoca
16
-
Propane-1,2-diol mutant T172A, pH not specified in the publication, temperature not specified in the publication Klebsiella oxytoca
24
-
Propane-1,2-diol mutant S224C, pH not specified in the publication, temperature not specified in the publication Klebsiella oxytoca
210
-
Propane-1,2-diol mutant T172S, pH not specified in the publication, temperature not specified in the publication Klebsiella oxytoca
430
-
Propane-1,2-diol mutant S224A, pH not specified in the publication, temperature not specified in the publication Klebsiella oxytoca
2200
-
Propane-1,2-diol wild-type, pH not specified in the publication, temperature not specified in the publication Klebsiella oxytoca