Literature summary for 4.2.1.3 extracted from
De Bellis, L.; Tsugeki, R.; Alpi, A.; Nishimura, M.
Purification and characterization of aconitase isoforms from etiolated pumpkin cotyledons (1993), Physiol. Plant., 99, 485-492.
No PubMed abstract available
General Stability
General Stability |
Organism |
freezing and thawing inactivates aconitase in absence of glycerol or sucrose |
Cucurbita sp. |
Inhibitors
Inhibitors |
Comment |
Organism |
Structure |
Fluorocitrate |
competitive |
Cucurbita sp. |
|
Localization
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
glyoxysome |
very low activity |
Cucurbita sp. |
9514 |
- |
Molecular Weight [Da]
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
89000 |
- |
1 * 89000, isoenzyme Aco I and Aco II, SDS-PAGE |
Cucurbita sp. |
100000 |
- |
isoenzyme Aco I and Aco II, gel filtration |
Cucurbita sp. |
Natural Substrates/ Products (Substrates)
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
additional information |
Cucurbita sp. |
enzyme is involved in the glyoxylate cycle |
? |
- |
? |
|
Organism
Organism |
UniProt |
Comment |
Textmining |
Cucurbita sp. |
- |
3 isoforms: Aco I, Aco II and Aco III |
- |
Purification (Commentary)
Purification (Comment) |
Organism |
isoenzymes Aco I and Aco II |
Cucurbita sp. |
Source Tissue
Source Tissue |
Comment |
Organism |
Textmining |
cotyledon |
etiolated |
Cucurbita sp. |
- |
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
2.126 |
- |
isoenzyme Aco II |
Cucurbita sp. |
2.214 |
- |
isoenzyme Aco I |
Cucurbita sp. |
Storage Stability
Storage Stability |
Organism |
-20°C, 25% w/v glycerol, stable for several weeks |
Cucurbita sp. |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
additional information |
enzyme is involved in the glyoxylate cycle |
Cucurbita sp. |
? |
- |
? |
|
Subunits
Subunits |
Comment |
Organism |
monomer |
1 * 89000, isoenzyme Aco I and Aco II, SDS-PAGE |
Cucurbita sp. |