Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | highly specific antagonistic effect of sodium versus potassium | Klebsiella pneumoniae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | causes inactivation by dissociation of the apoenzyme into its subunits. Inhibition is reversed by dialysis, Mg2+, glycerol or by addition of excess of either subunit | Klebsiella pneumoniae | |
N-ethylmaleimide | 1.0 mM blocks enzymatic reaction | Klebsiella pneumoniae | |
p-chloromercuribenzoate | 1.0 mM block enzymatic reaction | Klebsiella pneumoniae | |
salicylic acid | causes inactivation by dissociation of the apoenzyme into its subunits | Klebsiella pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.25 | - |
glycerol | in the presence of 0.1 M potassium phosphate buffer | Klebsiella pneumoniae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | required | Klebsiella pneumoniae | |
K+ | in the absence of other monovalent ions optimum K+ concentration: 0.08-0.15 M | Klebsiella pneumoniae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22000 | - |
gel filtration, subunit A, 20 mM potassium phosphate, pH 7.7, subunit A dissociates into sub-subunits of 11000 Da | Klebsiella pneumoniae |
90000 | - |
gel filtration, in the presence of ethanolamine buffer containing 0.1 M KCl, subunit B dissociates into sub-subunits | Klebsiella pneumoniae |
188000 | - |
sucrose density gradient centrifugation, inactive enzyme complex with hydroxocobalamin | Klebsiella pneumoniae |
189000 | - |
gel filtration in the presence of 50 mM ethanolamine-HCl buffer, pH 8.6, subunit B | Klebsiella pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerol | Klebsiella pneumoniae | - |
? | - |
? | |
glycerol | Klebsiella pneumoniae PZH 572 Warsaw | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | - |
available as Enterobacter aerogenes ATCC 25955 | - |
Klebsiella pneumoniae | - |
ATCC 25955 | - |
Klebsiella pneumoniae PZH 572 Warsaw | - |
available as Enterobacter aerogenes ATCC 25955 | - |
Oxidation Stability | Organism |
---|---|
holoenzyme can be protected from oxygen by addition of 50 mM K2SO3 | Klebsiella pneumoniae |
Purification (Comment) | Organism |
---|---|
apoenzyme, AB | Klebsiella pneumoniae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
228 | - |
apoenzyme | Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-propanediol | propane-1,2-diol | Klebsiella pneumoniae | propionaldehyde + H2O | - |
? | |
1,2-propanediol | propane-1,2-diol | Klebsiella pneumoniae PZH 572 Warsaw | propionaldehyde + H2O | - |
? | |
ethylene glycol | - |
Klebsiella pneumoniae | acetaldehyde + H2O | - |
? | |
ethylene glycol | - |
Klebsiella pneumoniae PZH 572 Warsaw | acetaldehyde + H2O | - |
? | |
glycerol | - |
Klebsiella pneumoniae | 3-hydroxypropanal + H2O | beta-hydroxypropionaldehyde | ? | |
glycerol | - |
Klebsiella pneumoniae PZH 572 Warsaw | 3-hydroxypropanal + H2O | beta-hydroxypropionaldehyde | ? | |
glycerol | - |
Klebsiella pneumoniae | ? | - |
? | |
glycerol | - |
Klebsiella pneumoniae PZH 572 Warsaw | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | the dissociation and association of the apoenzyme into subunits is influenced by pH, monovalent cations and glycerol | Klebsiella pneumoniae |
More | - |
Klebsiella pneumoniae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Klebsiella pneumoniae |
46 | - |
4 min, 50% destruction of the apoenzyme | Klebsiella pneumoniae |
70 | - |
inactivation of subunit A at 70°C is reversible | Klebsiella pneumoniae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.6 | - |
- |
Klebsiella pneumoniae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | enzyme activity between | Klebsiella pneumoniae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
5,6-Dimethylbenzimidazolylcobamide | coenzyme required | Klebsiella pneumoniae | |
5,6-dimethylbenzimidazolylcobamide 5'-phosphate | i.e. DBCC, the inactive enzyme complex with hydroxocobalamin can be converted to the active holoenzyme by replacement of hydroxocobalamin with DBCC in the presence of MG2+ and SO32- | Klebsiella pneumoniae |