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Literature summary for 4.2.1.30 extracted from

  • Johnson, B.C.; Stroinski, A.; Schneider, Z.
    Glycerol dehydratase from Aerobacter aerogenes (1975), Methods Enzymol., 42C, 315-323.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information highly specific antagonistic effect of sodium versus potassium Klebsiella pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
EDTA causes inactivation by dissociation of the apoenzyme into its subunits. Inhibition is reversed by dialysis, Mg2+, glycerol or by addition of excess of either subunit Klebsiella pneumoniae
N-ethylmaleimide 1.0 mM blocks enzymatic reaction Klebsiella pneumoniae
p-chloromercuribenzoate 1.0 mM block enzymatic reaction Klebsiella pneumoniae
salicylic acid causes inactivation by dissociation of the apoenzyme into its subunits Klebsiella pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.25
-
glycerol in the presence of 0.1 M potassium phosphate buffer Klebsiella pneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
K+ required Klebsiella pneumoniae
K+ in the absence of other monovalent ions optimum K+ concentration: 0.08-0.15 M Klebsiella pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22000
-
gel filtration, subunit A, 20 mM potassium phosphate, pH 7.7, subunit A dissociates into sub-subunits of 11000 Da Klebsiella pneumoniae
90000
-
gel filtration, in the presence of ethanolamine buffer containing 0.1 M KCl, subunit B dissociates into sub-subunits Klebsiella pneumoniae
188000
-
sucrose density gradient centrifugation, inactive enzyme complex with hydroxocobalamin Klebsiella pneumoniae
189000
-
gel filtration in the presence of 50 mM ethanolamine-HCl buffer, pH 8.6, subunit B Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycerol Klebsiella pneumoniae
-
?
-
?
glycerol Klebsiella pneumoniae PZH 572 Warsaw
-
?
-
?

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
available as Enterobacter aerogenes ATCC 25955
-
Klebsiella pneumoniae
-
ATCC 25955
-
Klebsiella pneumoniae PZH 572 Warsaw
-
available as Enterobacter aerogenes ATCC 25955
-

Oxidation Stability

Oxidation Stability Organism
holoenzyme can be protected from oxygen by addition of 50 mM K2SO3 Klebsiella pneumoniae

Purification (Commentary)

Purification (Comment) Organism
apoenzyme, AB Klebsiella pneumoniae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
228
-
apoenzyme Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-propanediol propane-1,2-diol Klebsiella pneumoniae propionaldehyde + H2O
-
?
1,2-propanediol propane-1,2-diol Klebsiella pneumoniae PZH 572 Warsaw propionaldehyde + H2O
-
?
ethylene glycol
-
Klebsiella pneumoniae acetaldehyde + H2O
-
?
ethylene glycol
-
Klebsiella pneumoniae PZH 572 Warsaw acetaldehyde + H2O
-
?
glycerol
-
Klebsiella pneumoniae 3-hydroxypropanal + H2O beta-hydroxypropionaldehyde ?
glycerol
-
Klebsiella pneumoniae PZH 572 Warsaw 3-hydroxypropanal + H2O beta-hydroxypropionaldehyde ?
glycerol
-
Klebsiella pneumoniae ?
-
?
glycerol
-
Klebsiella pneumoniae PZH 572 Warsaw ?
-
?

Subunits

Subunits Comment Organism
dimer the dissociation and association of the apoenzyme into subunits is influenced by pH, monovalent cations and glycerol Klebsiella pneumoniae
More
-
Klebsiella pneumoniae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
-
Klebsiella pneumoniae
46
-
4 min, 50% destruction of the apoenzyme Klebsiella pneumoniae
70
-
inactivation of subunit A at 70°C is reversible Klebsiella pneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.6
-
-
Klebsiella pneumoniae

pH Range

pH Minimum pH Maximum Comment Organism
8 9 enzyme activity between Klebsiella pneumoniae

Cofactor

Cofactor Comment Organism Structure
5,6-Dimethylbenzimidazolylcobamide coenzyme required Klebsiella pneumoniae
5,6-dimethylbenzimidazolylcobamide 5'-phosphate i.e. DBCC, the inactive enzyme complex with hydroxocobalamin can be converted to the active holoenzyme by replacement of hydroxocobalamin with DBCC in the presence of MG2+ and SO32- Klebsiella pneumoniae