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Literature summary for 4.2.1.30 extracted from

  • Seyfried, M; Daniel, R.; Gottschalk, G.
    Cloning, sequencing, and overexpression of the genes encoding coenzyme B12-dependent glycerol dehydratase of Citrobacter freundii (1996), J. Bacteriol., 178, 5793-5796.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.30

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Citrobacter freundii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Klebsiella pneumoniae
additional information
-
 additional information
-
 Limosilactobacillus reuteri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16121
-
 alpha2,beta2,gamma2, 2 * 60433 + 2 * 21487 + 2 * 16121, calculation from sequence of amino acid Citrobacter freundii
21487
-
 alpha2,beta2,gamma2, 2 * 60433 + 2 * 21487 + 2 * 16121, calculation from sequence of amino acid Citrobacter freundii
60433
-
 alpha2,beta2,gamma2, 2 * 60433 + 2 * 21487 + 2 * 16121, calculation from sequence of amino acid Citrobacter freundii
198000
-
 gel filtration, native enzyme Citrobacter freundii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycerol Klebsiella pneumoniae
-
 ?
-
 ?
glycerol Citrobacter freundii
-
 ?
-
 ?
glycerol Limosilactobacillus reuteri
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Citrobacter freundii
-
-
-
Klebsiella pneumoniae
-
-
-
Limosilactobacillus reuteri
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycerol
-
 Klebsiella pneumoniae 3-hydroxypropanal + H2O
-
 ?
glycerol
-
 Citrobacter freundii 3-hydroxypropanal + H2O
-
 ?
glycerol
-
 Limosilactobacillus reuteri 3-hydroxypropanal + H2O
-
 ?
glycerol
-
 Klebsiella pneumoniae ?
-
 ?
glycerol
-
 Citrobacter freundii ?
-
 ?
glycerol
-
 Limosilactobacillus reuteri ?
-
 ?

Subunits

Subunits Comment Organism
hexamer alpha2,beta2,gamma2, 2 * 60433 + 2 * 21487 + 2 * 16121, calculation from sequence of amino acid Citrobacter freundii

Cofactor

Cofactor Comment Organism Structure
5,6-Dimethylbenzimidazolylcobamide coenzyme required Klebsiella pneumoniae
5,6-Dimethylbenzimidazolylcobamide coenzyme required Citrobacter freundii
5,6-Dimethylbenzimidazolylcobamide coenzyme required Limosilactobacillus reuteri