Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | is critical to the reactivation of glycerol dehydratase | Klebsiella pneumoniae | |
coenzyme B12 | is critical to the reactivation of glycerol dehydratase | Klebsiella pneumoniae |
Cloned (Comment) | Organism |
---|---|
plasmid PKRX-T bearing the gene of the dhaBCE double digested with EcoR I and Sac I, resulting 2.7 kb fragment containing the intact dhaBCE genes inserted into pET-28a (+) vector linearized with the same enzymes. Transformation of the ligation mixture (pET-28 (+) dhaBCE) into Escherichia coli BL21 (DE3) for overexpression | Klebsiella pneumoniae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glycerol | can inactive the glycerol dehydratase expressed in Escherichia coli quickly in 10 min. Can be effectively reactivated to 98.9% by 50 mM ATP, 10 mM Mg2+ and 0.003 mM coenzyme B12, respectively, when the ratio (w/w) of glycerol dehydratase to reactivation factor is 4:1. Activity of glycerol inactivated GDHt is easier to be recovered than that of O2-inactivated GDHt | Klebsiella pneumoniae | |
O2 | can inactive the glycerol dehydratase expressed in Escherichia coli quickly in 10 min. Can be effectively reactivated to 97.3% by 50 mM ATP, 10 mM Mg2+ and 0.003 mM coenzyme B12, respectively, when the ratio (w/w) of glycerol dehydratase to reactivation factor is 4:1. Activity of glycerol inactivated GDHt is easier to be recovered than that of O2-inactivated GDHt | Klebsiella pneumoniae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Klebsiella pneumoniae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16101 | - |
x * 60702 + x * 21322 + x * 16101, alphabetagamma, sequence analysis | Klebsiella pneumoniae |
21322 | - |
x * 60702 + x * 21322 + x * 16101, alphabetagamma, sequence analysis | Klebsiella pneumoniae |
60702 | - |
x * 60702 + x * 21322 + x * 16101, alphabetagamma, sequence analysis | Klebsiella pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | Q59475 and A6NA24 and A6NA25 | Q59475: small subunit, A6NA24: large subunit, A6NA25: small subunit | - |
Klebsiella pneumoniae XJPD-Li | Q59475 and A6NA24 and A6NA25 | Q59475: small subunit, A6NA24: large subunit, A6NA25: small subunit | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.299 | - |
recombined glycerol dehydratase | Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-propanediol | - |
Klebsiella pneumoniae | propionaldehyde + H2O | - |
? | |
1,2-propanediol | - |
Klebsiella pneumoniae XJPD-Li | propionaldehyde + H2O | - |
? | |
glycerol | - |
Klebsiella pneumoniae | 3-hydroxypropanal + H2O | - |
? | |
glycerol | - |
Klebsiella pneumoniae XJPD-Li | 3-hydroxypropanal + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60702 + x * 21322 + x * 16101, alphabetagamma, sequence analysis | Klebsiella pneumoniae |
Synonyms | Comment | Organism |
---|---|---|
DhaBCE | - |
Klebsiella pneumoniae |
GDHt | - |
Klebsiella pneumoniae |
glycerol dehydratase | - |
Klebsiella pneumoniae |
General Information | Comment | Organism |
---|---|---|
physiological function | overexpressed glycerol dehydratase shows higher enzyme activity than that of wild-strain | Klebsiella pneumoniae |