Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109 cells | Citrobacter freundii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
Propane-1,2-diol | at pH 8.5 and 45°C | Citrobacter freundii | |
0.59 | - |
glycerol | at pH 8.5 and 45°C | Citrobacter freundii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16000 | - |
x * 61000 + x * 22000 + x * 16000, SDS-PAGE | Citrobacter freundii |
22000 | - |
x * 61000 + x * 22000 + x * 16000, SDS-PAGE | Citrobacter freundii |
61000 | - |
x * 61000 + x * 22000 + x * 16000, SDS-PAGE | Citrobacter freundii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerol | Citrobacter freundii | best substrate | 3-hydroxypropanal + H2O | - |
? | |
glycerol | Citrobacter freundii AS1.1732 | best substrate | 3-hydroxypropanal + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrobacter freundii | A8CH84 and A8CH91 and A8CH95 | large, middle and small subunit | - |
Citrobacter freundii AS1.1732 | A8CH84 and A8CH91 and A8CH95 | large, middle and small subunit | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography and Sephacryl S-300 gel filtration | Citrobacter freundii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
21.2 | - |
crude extract, at pH 8.5 and 45°C | Citrobacter freundii |
91.9 | - |
after 4.3fold purification, at pH 8.5 and 45°C | Citrobacter freundii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerol | best substrate | Citrobacter freundii | 3-hydroxypropanal + H2O | - |
? | |
glycerol | best substrate | Citrobacter freundii AS1.1732 | 3-hydroxypropanal + H2O | - |
? | |
propane-1,2-diol | - |
Citrobacter freundii | ? | - |
? | |
propane-1,2-diol | - |
Citrobacter freundii AS1.1732 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 61000 + x * 22000 + x * 16000, SDS-PAGE | Citrobacter freundii |
Synonyms | Comment | Organism |
---|---|---|
DhaB | large subunit | Citrobacter freundii |
DhaC | middle subunit | Citrobacter freundii |
DhaE | small subunit | Citrobacter freundii |
GDHt | - |
Citrobacter freundii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
activity is lost dramatically at temperatures above 45°C | Citrobacter freundii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Citrobacter freundii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9.5 | the relative enzyme activity at pH values ranging from 7.0 to 9.5 is over 80%. Activity drops dramatically when the pH value is higher than 9.5 | Citrobacter freundii |