Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.1.30 extracted from

  • Qi, X.; Guo, Q.; Wei, Y.; Xu, H.; Huang, R.
    Enhancement of pH stability and activity of glycerol dehydratase from Klebsiella pneumoniae by rational design (2012), Biotechnol. Lett., 34, 339-346.
    View publication on PubMed
Search for more literature for 4.2.1.30

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli XL10-Gold cells Klebsiella pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 pentane-1,2-diol mutant enzyme KpG525, at pH 6.0 and 37°C Klebsiella pneumoniae
0.2
-
 pentane-1,2-diol mutant enzyme KpG60, at pH 6.0 and 37°C Klebsiella pneumoniae
0.24
-
 pentane-1,2-diol wild type enzyme, at pH 6.0 and 37°C Klebsiella pneumoniae
0.6
-
 glycerol wild type enzyme, at pH 6.0 and 37°C Klebsiella pneumoniae
0.6
-
 glycerol mutant enzyme KpG525, at pH 6.0 and 37°C Klebsiella pneumoniae
0.7
-
 glycerol mutant enzyme KpG60, at pH 6.0 and 37°C Klebsiella pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16000
-
 2 * 62000 + 2 * 22000 + 2 * 16000, SDS-PAGE Klebsiella pneumoniae
22000
-
 2 * 62000 + 2 * 22000 + 2 * 16000, SDS-PAGE Klebsiella pneumoniae
62000
-
 2 * 62000 + 2 * 22000 + 2 * 16000, SDS-PAGE Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycerol Klebsiella pneumoniae
-
 3-hydroxypropanal + H2O
-
 ?
glycerol Klebsiella pneumoniae 10018
-
 3-hydroxypropanal + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae A8CIU9 large subunit
-
Klebsiella pneumoniae 10018 A8CIU9 large subunit
-

Purification (Commentary)

Purification (Comment) Organism
Ni-nitrilotriacetate agarose affinity column chromatography and Sephacryl S-300 gel filtration Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycerol
-
 Klebsiella pneumoniae 3-hydroxypropanal + H2O
-
 ?
glycerol
-
 Klebsiella pneumoniae 10018 3-hydroxypropanal + H2O
-
 ?
pentane-1,2-diol
-
 Klebsiella pneumoniae ?
-
 ?
pentane-1,2-diol
-
 Klebsiella pneumoniae 10018 ?
-
 ?

Subunits

Subunits Comment Organism
heterohexamer 2 * 62000 + 2 * 22000 + 2 * 16000, SDS-PAGE Klebsiella pneumoniae

Synonyms

Synonyms Comment Organism
GDHt
-
 Klebsiella pneumoniae
KpG
-
 Klebsiella pneumoniae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Klebsiella pneumoniae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
200
-
 pentane-1,2-diol wild type enzyme, at pH 6.0 and 37°C Klebsiella pneumoniae
250
-
 pentane-1,2-diol mutant enzyme KpG60, at pH 6.0 and 37°C Klebsiella pneumoniae
310
-
 pentane-1,2-diol mutant enzyme KpG525, at pH 6.0 and 37°C Klebsiella pneumoniae
430
-
 glycerol wild type enzyme, at pH 6.0 and 37°C Klebsiella pneumoniae
460
-
 glycerol mutant enzyme KpG60, at pH 6.0 and 37°C Klebsiella pneumoniae
890
-
 glycerol mutant enzyme KpG525, at pH 6.0 and 37°C Klebsiella pneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Klebsiella pneumoniae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
660
-
 glycerol mutant enzyme KpG60, at pH 6.0 and 37°C Klebsiella pneumoniae
710
-
 glycerol wild type enzyme, at pH 6.0 and 37°C Klebsiella pneumoniae
840
-
 pentane-1,2-diol wild type enzyme, at pH 6.0 and 37°C Klebsiella pneumoniae
1200
-
 pentane-1,2-diol mutant enzyme KpG60, at pH 6.0 and 37°C Klebsiella pneumoniae
1400
-
 glycerol mutant enzyme KpG525, at pH 6.0 and 37°C Klebsiella pneumoniae
1500
-
 pentane-1,2-diol mutant enzyme KpG525, at pH 6.0 and 37°C Klebsiella pneumoniae