Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement | Salmonella enterica subsp. enterica serovar Typhimurium | |
additional information | no requirement | Neurospora crassa |
General Stability | Organism |
---|---|
10% inactivation at 0°C in phosphate buffer at pH 7 or above | Neurospora crassa |
loss of activity after repeated freezing and thawing | Neurospora crassa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
beta-carboxy-beta-hydroxyisocaproate | synthetic, racemic mixture | Neurospora crassa | |
beta-carboxy-beta-hydroxyisocaproate | synthetic, racemic mixture | Salmonella enterica subsp. enterica serovar Typhimurium | |
cysteine | 55% inhibition at 20 mM, protected by substrate | Neurospora crassa | |
cysteine | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
glutathione | protection by substrate | Neurospora crassa | |
glutathione | protection by substrate | Salmonella enterica subsp. enterica serovar Typhimurium | |
mercaptoethanol | protection by substrate | Neurospora crassa | |
mercaptoethanol | protection by substrate | Salmonella enterica subsp. enterica serovar Typhimurium | |
additional information | additional compounds tested, without effect | Neurospora crassa | |
additional information | additional compounds tested, without effect | Salmonella enterica subsp. enterica serovar Typhimurium | |
N-ethylmaleimide | - |
Neurospora crassa | |
N-ethylmaleimide | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
sulfhydryl compounds | protection by substrate | Neurospora crassa | |
sulfhydryl compounds | protection by substrate | Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.078 | - |
alpha-hydroxy-beta-carboxyisocaproate | - |
Neurospora crassa | |
0.18 | - |
dimethylcitraconate | - |
Neurospora crassa | |
0.95 | - |
beta-carboxy-beta-hydroxyisocaproate | - |
Neurospora crassa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement | Salmonella enterica subsp. enterica serovar Typhimurium | |
additional information | no requirement | Neurospora crassa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | 90000 | sucrose density gradient centrifugation | Neurospora crassa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neurospora crassa | - |
- |
- |
Salmonella enterica subsp. enterica serovar Typhimurium | - |
wild type and Leu- -mutants | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(2R,3S)-3-isopropylmalate = 2-isopropylmaleate + H2O | equilibrium concentration ratios | Neurospora crassa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.2 | - |
- |
Neurospora crassa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-isopropylmaleate + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 3-isopropylmalate | - |
? | |
2-isopropylmaleate + H2O | - |
Neurospora crassa | 3-isopropylmalate | - |
? | |
3-isopropylmalate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 2-isopropylmaleate + H2O | - |
? | |
3-isopropylmalate | - |
Neurospora crassa | 2-isopropylmaleate + H2O | - |
? | |
beta-carboxy-beta-hydroxyisocaproate | - |
Neurospora crassa | ? | - |
? | |
dimethylcitraconate | - |
Neurospora crassa | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
32 | - |
enzyme assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
32 | - |
enzyme assay at | Neurospora crassa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
50% loss of activity after 2 min | Neurospora crassa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
7 | - |
- |
Neurospora crassa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | at pH 6.0, 20% of optimum activity, activity varies only about 20% between pH 7.0 and 8.0 | Salmonella enterica subsp. enterica serovar Typhimurium |
6 | 8 | at pH 6.0, 20% of optimum activity, activity varies only about 20% between pH 7.0 and 8.0 | Neurospora crassa |