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Literature summary for 4.2.1.33 extracted from

  • Gross, S.R.; Umbarger, H.E.
    The biosynthesis of leucine: II. The enzymic isomerization of beta-carboxy-beta-hydroxyisocaproate and alpha-hydroxy-beta-carboxyisocaproate (1963), Biochemistry, 2, 1046-1052.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information no requirement Salmonella enterica subsp. enterica serovar Typhimurium
additional information no requirement Neurospora crassa

General Stability

General Stability Organism
10% inactivation at 0°C in phosphate buffer at pH 7 or above Neurospora crassa
loss of activity after repeated freezing and thawing Neurospora crassa

Inhibitors

Inhibitors Comment Organism Structure
beta-carboxy-beta-hydroxyisocaproate synthetic, racemic mixture Neurospora crassa
beta-carboxy-beta-hydroxyisocaproate synthetic, racemic mixture Salmonella enterica subsp. enterica serovar Typhimurium
cysteine 55% inhibition at 20 mM, protected by substrate Neurospora crassa
cysteine
-
Salmonella enterica subsp. enterica serovar Typhimurium
glutathione protection by substrate Neurospora crassa
glutathione protection by substrate Salmonella enterica subsp. enterica serovar Typhimurium
mercaptoethanol protection by substrate Neurospora crassa
mercaptoethanol protection by substrate Salmonella enterica subsp. enterica serovar Typhimurium
additional information additional compounds tested, without effect Neurospora crassa
additional information additional compounds tested, without effect Salmonella enterica subsp. enterica serovar Typhimurium
N-ethylmaleimide
-
Neurospora crassa
N-ethylmaleimide
-
Salmonella enterica subsp. enterica serovar Typhimurium
sulfhydryl compounds protection by substrate Neurospora crassa
sulfhydryl compounds protection by substrate Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.078
-
alpha-hydroxy-beta-carboxyisocaproate
-
Neurospora crassa
0.18
-
dimethylcitraconate
-
Neurospora crassa
0.95
-
beta-carboxy-beta-hydroxyisocaproate
-
Neurospora crassa

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no requirement Salmonella enterica subsp. enterica serovar Typhimurium
additional information no requirement Neurospora crassa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000 90000 sucrose density gradient centrifugation Neurospora crassa

Organism

Organism UniProt Comment Textmining
Neurospora crassa
-
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
-
wild type and Leu- -mutants
-

Reaction

Reaction Comment Organism Reaction ID
(2R,3S)-3-isopropylmalate = 2-isopropylmaleate + H2O equilibrium concentration ratios Neurospora crassa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2
-
-
Neurospora crassa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-isopropylmaleate + H2O
-
Salmonella enterica subsp. enterica serovar Typhimurium 3-isopropylmalate
-
?
2-isopropylmaleate + H2O
-
Neurospora crassa 3-isopropylmalate
-
?
3-isopropylmalate
-
Salmonella enterica subsp. enterica serovar Typhimurium 2-isopropylmaleate + H2O
-
?
3-isopropylmalate
-
Neurospora crassa 2-isopropylmaleate + H2O
-
?
beta-carboxy-beta-hydroxyisocaproate
-
Neurospora crassa ?
-
?
dimethylcitraconate
-
Neurospora crassa ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
32
-
enzyme assay at Salmonella enterica subsp. enterica serovar Typhimurium
32
-
enzyme assay at Neurospora crassa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
50% loss of activity after 2 min Neurospora crassa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
7
-
-
Neurospora crassa

pH Range

pH Minimum pH Maximum Comment Organism
6 8 at pH 6.0, 20% of optimum activity, activity varies only about 20% between pH 7.0 and 8.0 Salmonella enterica subsp. enterica serovar Typhimurium
6 8 at pH 6.0, 20% of optimum activity, activity varies only about 20% between pH 7.0 and 8.0 Neurospora crassa