Cloned (Comment) | Organism |
---|---|
expression of FLAG-tagged LeuC and of the three LeuD isoforms in transgenic Arabidosis thaliana plants, interaction analysis, overview | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of AtLeuD1 T-DNA knockout mutant and AtLeuD -RNAi plants, i.e. specific AtLeuD2 -RNAi, AtLeuD -RNAi, atleud1 /AtLeuD2 -RNAi and atleud1 /AtLeuD -RNAi. Lethal phenotype of the atleud3 mutant | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast stroma | - |
Arabidopsis thaliana | 9570 | - |
additional information | analysis of subcellular localization of AtLeuC and AtLeuDs within Arabidopsis chloroplast compartments, overview | Arabidopsis thaliana | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
the Arabidopsis genome encodes one potential large subunit and three potential small subunits, encoded by AtleuC and AtleuD genes | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | expression in all floral tissues including sepal, stamen, pistil as well as pollen grains, but it is only present at both ends of developing siliques | Arabidopsis thaliana | - |
leaf | - |
Arabidopsis thaliana | - |
additional information | semi-quantitative RT-PCR analysis of temporal and spatial expression of AtLeuC and AtLeuD genes, overview. The tissue-specifis expression analysis reveals that the patterns of small subunits AtLeuD1 and AtLeuD2 expression are similar, but distinct from that of AtLeuD3 | Arabidopsis thaliana | - |
root | - |
Arabidopsis thaliana | - |
Synonyms | Comment | Organism |
---|---|---|
IPMI | - |
Arabidopsis thaliana |
isopropylmalate isomerase | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | lethal phenotype of the atleud3 mutant with a defect in female gametophyte development | Arabidopsis thaliana |
metabolism | isopropylmalate isomerase large subunit and small subunits form heterodimers to catalyze the isomerization of 2-isopropylmalate to 3-isopropylmalate in leucine biosynthesis in bacteria and archaea. Reverse genetics and metabolite profiling show that AtLeuD1 and AtLeuD2 function redundantly in aliphatic glucosinolate biosynthesis, but AtLeuD3 is not likely to be involved in this pathway | Arabidopsis thaliana |
physiological function | isopropylmalate isomerase large subunit and small subunits form heterodimers to catalyze the isomerization of 2-isopropylmalate to 3-isopropylmalate in leucine biosynthesis in bacteria and archaea. AtLeuD3 plays an essential role in leucine biosynthesis and female gametophyte development | Arabidopsis thaliana |