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Literature summary for 4.2.1.33 extracted from

  • Lee, E.H.; Cho, Y.W.; Hwang, K.Y.
    Crystal structure of LeuD from Methanococcus jannaschii (2012), Biochem. Biophys. Res. Commun., 419, 160-164.
    View publication on PubMed
Search for more literature for 4.2.1.33

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Methanocaldococcus jannaschii

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 25% (w/v) polyethylene glycol monomethyl ether 2000 and 0.1 MTris-HCl (pH 7.9) Methanocaldococcus jannaschii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18000
-
 x * 46000 + x * 18000, SDS-PAGE Methanocaldococcus jannaschii
46000
-
 x * 46000 + x * 18000, SDS-PAGE Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2R,3S)-3-isopropylmalate Methanocaldococcus jannaschii
-
 2-isopropylmaleate + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii Q58673
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel-affinity column chromatography and Superdex 200 gel filtration Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,3S)-3-isopropylmalate
-
 Methanocaldococcus jannaschii 2-isopropylmaleate + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 46000 + x * 18000, SDS-PAGE Methanocaldococcus jannaschii

Synonyms

Synonyms Comment Organism
3-isopropylmalate isomerase
-
 Methanocaldococcus jannaschii
IPM isomerase
-
 Methanocaldococcus jannaschii
isopropylmalate isomerase
-
 Methanocaldococcus jannaschii
leuC large subunit Methanocaldococcus jannaschii
LeuD small subunit Methanocaldococcus jannaschii