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Literature summary for 4.2.1.41 extracted from

  • Dagley, S.; Jeffcoat, R.
    D-4-Deoxy-5-ketoglucarate hydro-lyase (decarboxylating) (1975), Methods Enzymol., 42C, 272-276.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide inhibition after prolonged incubation Delftia acidovorans
iodoacetate inhibition after prolonged incubation Delftia acidovorans
p-chloromercuribenzene sulfonic acid
-
Delftia acidovorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
118000
-
sedimentation equilibrium Delftia acidovorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-dehydro-4-deoxy-D-glucarate Pseudomonas sp.
-
?
-
?
5-dehydro-4-deoxy-D-glucarate Delftia acidovorans
-
?
-
?

Organism

Organism UniProt Comment Textmining
Delftia acidovorans
-
ATCC 17455
-
Pseudomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Delftia acidovorans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.9
-
-
Delftia acidovorans

Storage Stability

Storage Stability Organism
-15°C, several weeks Delftia acidovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-dehydro-4-deoxy-D-glucarate D-4-deoxy-5-ketoglucarate Pseudomonas sp. 2,5-dioxopentanoate + H2O + CO2 2,5-diketovalerate ?
5-dehydro-4-deoxy-D-glucarate D-4-deoxy-5-ketoglucarate Delftia acidovorans 2,5-dioxopentanoate + H2O + CO2 2,5-diketovalerate ?
5-dehydro-4-deoxy-D-glucarate
-
Pseudomonas sp. ?
-
?
5-dehydro-4-deoxy-D-glucarate
-
Delftia acidovorans ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.3
-
Delftia acidovorans

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8 sharp decrease in activity below pH 6.5 and above pH 8 Delftia acidovorans