Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | irreversible | Escherichia coli | |
p-hydroxymercuribenzoate | complete inhibition after incubation with 10 mM for 15 min at 25¦C, recovery of activity with 2-mercaptoethanol | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
dTDPglucose | - |
Escherichia coli | |
2.2 | - |
dUDPglucose | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
78000 | - |
sedimentation equilibrium centrifugation | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-glucose | Escherichia coli | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.2 | - |
dTDPglucose | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-glucose | - |
Escherichia coli | dTDP-4-dehydro-6-deoxy-D-glucose + H2O | - |
? | |
dTDP-glucose | - |
Escherichia coli | ? | - |
? | |
dUDP-glucose | - |
Escherichia coli | dUDP-4-dehydro-6-deoxy-D-glucose + H2O | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | dTDPglucose | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | at pH 7.0 50% of activity at pH 8.0, at pH 9.0 90% of activity at pH 8.0 | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | 1 mol of bound NAD+ per mol of enzyme | Escherichia coli |